PDBsum entry 4x08

Hydrolase

PDB id: 4x08

Contents

Protein chains

133 a.a.

Ligands

SO4 ×16

Waters ×422

PDB id:

4x08

Name:

Hydrolase

Title:

Structure of h128n/ecp mutant in complex with sulphate anions at 1.34 angstroms.

Structure:

Eosinophil cationic protein. Chain: a, b. Synonym: ecp,ribonuclease 3,rnase 3. Engineered: yes. Mutation: yes. Other_details: residue 97 corresponds to a protein natural variant

Source:

Homo sapiens. Human. Organism_taxid: 9606. Organ: bone marrow. Cell: eosinophils. Gene: rnase3, ecp, rns3. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.34Å R-factor: 0.186 R-free: 0.218

Authors:

J.A.Blanco,J.M.Garcia,V.A.Salazar,D.Sanchez,M.Moussauoi,E.Boix

Key ref:

J.A.Blanco et al. Structure of h128n/ecp mutant in complex with sulphat anions at 1.34 angstroms.. To be published, .

Date:

21-Nov-14 Release date: 07-Oct-15

Protein chains

P12724  (ECP_HUMAN) -  Eosinophil cationic protein from Homo sapiens

Seq: 160 a.a.

Struc: 133 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.1.27.- - ?????