PDBsum entry 4v2c

Apoptosis

PDB id: 4v2c

Contents

Protein chains

323 a.a.

106 a.a.

PDB id:

4v2c

Name:

Apoptosis

Title:

Mouse flrt2 lrr domain in complex with rat unc5d ig1 domain

Structure:

Fibronectin leucine rich transmembrane protein 2. Chain: a, c. Fragment: lrr domain, residues 35-362. Synonym: protein flrt2. Engineered: yes. Protein unc5d. Chain: b, d. Fragment: ig1 domain, residues 1-161. Engineered: yes

Source:

Mus musculus. House mouse. Organism_taxid: 10090. Expressed in: homo sapiens. Expression_system_taxid: 9606. Expression_system_cell_line: hek293t. Rattus norvegicus. Norway rat. Organism_taxid: 10116.

Resolution:

4.00Å R-factor: 0.319 R-free: 0.335

Authors:

E.Seiradake,D.Del Toro,D.Nagel,F.Cop,R.Haertl,T.Ruff,G.Seyit-Bremer, K.Harlos,E.C.Border,A.Acker-Palmer,E.Y.Jones,R.Klein

Key ref:

E.Seiradake et al. (2014). FLRT structure: balancing repulsion and cell adhesion in cortical and vascular development. Neuron, 84, 370-385. PubMed id: 25374360 DOI: 10.1016/j.neuron.2014.10.008

Date:

08-Oct-14 Release date: 05-Nov-14

Protein chains

Q8BLU0  (FLRT2_MOUSE) -  Leucine-rich repeat transmembrane protein FLRT2 from Mus musculus

Seq: 660 a.a.

Struc: 323 a.a.

Protein chains

F1LW30  (UNC5D_RAT) -  Netrin receptor UNC5D from Rattus norvegicus

Seq: 956 a.a.

Struc: 106 a.a.

DOI no: 10.1016/j.neuron.2014.10.008

Neuron 84:370-385 (2014)

PubMed id: 25374360

FLRT structure: balancing repulsion and cell adhesion in cortical and vascular development.

E.Seiradake, D.del Toro, D.Nagel, F.Cop, R.Härtl, T.Ruff, G.Seyit-Bremer, K.Harlos, E.C.Border, A.Acker-Palmer, E.Y.Jones, R.Klein.

ABSTRACT

FLRTs are broadly expressed proteins with the unique property of acting as homophilic cell adhesion molecules and as heterophilic repulsive ligands of Unc5/Netrin receptors. How these functions direct cell behavior and the molecular mechanisms involved remain largely unclear. Here we use X-ray crystallography to reveal the distinct structural bases for FLRT-mediated cell adhesion and repulsion in neurons. We apply this knowledge to elucidate FLRT functions during cortical development. We show that FLRTs regulate both the radial migration of pyramidal neurons, as well as their tangential spread. Mechanistically, radial migration is controlled by repulsive FLRT2-Unc5D interactions, while spatial organization in the tangential axis involves adhesive FLRT-FLRT interactions. Further, we show that the fundamental mechanisms of FLRT adhesion and repulsion are conserved between neurons and vascular endothelial cells. Our results reveal FLRTs as powerful guidance factors with structurally encoded repulsive and adhesive surfaces.