PDBsum entry 4roh

Ligase

PDB id: 4roh

Contents

Protein chains

76 a.a.

14 a.a.

13 a.a.

Ligands

UNX ×11

Waters ×61

Superseded by:

5cq2

PDB id:

4roh

Name:

Ligase

Title:

Crystal structure of tandem ww domains of itch in complex with txnip peptide

Structure:

E3 ubiquitin-protein ligase itchy homolog. Chain: a. Fragment: unp residues 433-521. Synonym: itch, atrophin-1-interacting protein 4, aip4, nfe2- associated polypeptide 1, napp1. Engineered: yes. Thioredoxin-interacting protein. Chain: b, c. Fragment: unp residues 327-338.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: itch. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Organism_taxid: 9606

Resolution:

1.34Å R-factor: 0.161 R-free: 0.189

Authors:

Y.Liu,W.Tempel,C.Bountra,C.H.Arrowsmith,A.M.Edwards,J.Min,Structural Genomics Consortium (Sgc)

Key ref:

Y.Liu et al. Crystal structure of tandem ww domains of itch in com with txnip peptide. To be published, .

Date:

28-Oct-14 Release date: 10-Dec-14

Protein chain

Q96J02  (ITCH_HUMAN) -  E3 ubiquitin-protein ligase Itchy homolog from Homo sapiens

Seq: 903 a.a.

Struc: 76 a.a.

Protein chain

Q9H3M7  (TXNIP_HUMAN) -  Thioredoxin-interacting protein from Homo sapiens

Seq: 391 a.a.

Struc: 13 a.a.*

Protein chain

Q9H3M7  (TXNIP_HUMAN) -  Thioredoxin-interacting protein from Homo sapiens

Seq: 391 a.a.

Struc: 13 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.6.3.2.- - ?????