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PDBsum entry 4mxf

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protein metals Protein-protein interface(s) links
Hydrolase PDB id
4mxf
Jmol
Contents
Protein chains
124 a.a.
Metals
_AU
Waters ×100
PDB id:
4mxf
Name: Hydrolase
Title: X-ray structure of the adduct between bovine pancreatic ribo and auoxo6, a dinuclear gold(iii) complex with -dioxo bridg the two metal centers
Structure: Ribonuclease pancreatic. Chain: a, b. Synonym: rnase 1, rnase a. Engineered: yes
Source: Bos taurus. Bovine,cow,domestic cattle,domestic cow. Organism_taxid: 9913. Gene: rnase1, rns1. Expressed in: escherichia coli. Expression_system_taxid: 562
Resolution:
2.25Å     R-factor:   0.189     R-free:   0.268
Authors: I.Russo Krauss,A.Vergara,A.Merlino
Key ref: L.Messori et al. (2014). Interactions of gold-based drugs with proteins: crystal structure of the adduct formed between ribonuclease A and a cytotoxic gold(III) compound. Metallomics, 6, 233-236. PubMed id: 24287583 DOI: 10.1039/c3mt00265a
Date:
26-Sep-13     Release date:   25-Dec-13    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P61823  (RNAS1_BOVIN) -  Ribonuclease pancreatic
Seq:
Struc:
150 a.a.
124 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.3.1.27.5  - Pancreatic ribonuclease.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in C-P or U-P with 2',3'-cyclic phosphate intermediates.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   1 term 
  Biological process     metabolic process   3 terms 
  Biochemical function     nucleic acid binding     6 terms  

 

 
DOI no: 10.1039/c3mt00265a Metallomics 6:233-236 (2014)
PubMed id: 24287583  
 
 
Interactions of gold-based drugs with proteins: crystal structure of the adduct formed between ribonuclease A and a cytotoxic gold(III) compound.
L.Messori, F.Scaletti, L.Massai, M.A.Cinellu, I.Russo Krauss, G.di Martino, A.Vergara, L.Paduano, A.Merlino.
 
  ABSTRACT  
 
The reaction of Auoxo6, a dinuclear gold(iii) complex, with the model protein bovine pancreatic ribonuclease is explored here by X-ray diffraction and ESI mass spectrometry. Data provide clues on the processes of adduct formation and of enzyme inhibition and, inductively, on the likely mode of action of this metallodrug.