PDBsum entry 4lx5

RNA

PDB id: 4lx5

Contents

DNA/RNA

Ligands

29G

Metals

_MG ×4

Waters ×17

PDB id:

4lx5

Name:

RNA

Title:

X-ray crystal structure of the m6" riboswitch aptamer bound to pyrimido[4,5-d]pyrimidine-2,4-diamine (ppda)

Structure:

Mutated adenine riboswitch aptamer. Chain: a. Engineered: yes. Mutation: yes

Source:

Synthetic: yes. Vibrio vulnificus. Organism_taxid: 672. Other_details: in vitro transcription

Resolution:

2.13Å R-factor: 0.202 R-free: 0.257

Authors:

M.S.Dunstan,D.Leys

Key ref:

C.J.Robinson et al. (2014). Modular riboswitch toolsets for synthetic genetic control in diverse bacterial species. J Am Chem Soc, 136, 10615-10624. PubMed id: 24971878 DOI: 10.1021/ja502873j

Date:

29-Jul-13 Release date: 16-Jul-14

DNA/RNA chain

G-G-C-U-U-C-A-U-A-U-A-A-U-C-C-G-A-A-U-G-A-U-A-U-G-G-U-U-U-C-G-G-A-G-C-U-U-C-C- 71 bases

DOI no: 10.1021/ja502873j

J Am Chem Soc 136:10615-10624 (2014)

PubMed id: 24971878

Modular riboswitch toolsets for synthetic genetic control in diverse bacterial species.

C.J.Robinson, H.A.Vincent, M.C.Wu, P.T.Lowe, M.S.Dunstan, D.Leys, J.Micklefield.

ABSTRACT

Ligand-dependent control of gene expression is essential for gene functional analysis, target validation, protein production, and metabolic engineering. However, the expression tools currently available are difficult to transfer between species and exhibit limited mechanistic diversity. Here we demonstrate how the modular architecture of purine riboswitches can be exploited to develop orthogonal and chimeric switches that are transferable across diverse bacterial species, modulating either transcription or translation, to provide tunable activation or repression of target gene expression, in response to synthetic non-natural effector molecules. Our novel riboswitch-ligand pairings are shown to regulate physiologically important genes required for bacterial motility in Escherichia coli and cell morphology in Bacillus subtilis. These findings are relevant for future gene function studies and antimicrobial target validation, while providing new modular and orthogonal regulatory components for deployment in synthetic biology regimes.