PDBsum entry 4kpe

Isomerase/DNA/inhibitor

PDB id: 4kpe

Contents

Protein chains

482 a.a.

208 a.a.

DNA/RNA

Ligands

AF5 ×2

Metals

_MG ×6

Waters ×24

PDB id:

4kpe

Name:

Isomerase/DNA/inhibitor

Title:

Novel fluoroquinolones in complex with topoisomerase iv from s. Pneumoniae and e-site g-gate

Structure:

DNA topoisomerase 4 subunit a. Chain: a, b. Fragment: parc55, unp residues 1-488. Synonym: topoisomerase iv subunit a. Engineered: yes. Mutation: yes. DNA topoisomerase 4 subunit b. Chain: c, d. Fragment: pare30, unp residues 404-647.

Source:

Streptococcus pneumoniae. Organism_taxid: 170187. Strain: atcc baa-334 / tigr4. Gene: parc, sp_0855. Expressed in: escherichia coli. Expression_system_taxid: 562. Streptococcus pneumoniae serotype 4. Gene: pare. Synthetic: yes.

Resolution:

3.43Å R-factor: 0.156 R-free: 0.195

Authors:

I.Laponogov,X.-S.Pan,D.A.Vesekov,R.T.Cirz,A.S.Wagman,H.E.Moser, L.M.Fisher,M.R.Sanderson

Key ref:

I.Laponogov et al. Novel fluoroquinolones in complex with topoisomerase s. Pneumoniae and e-Site g-Gate. To be published, . PubMed id: 27655731

Date:

13-May-13 Release date: 26-Nov-14

Protein chains

P72525  (PARC_STRPN) -  DNA topoisomerase 4 subunit A from Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)

Seq: 823 a.a.

Struc: 482 a.a.*

Protein chains

Q59961  (PARE_STRPN) -  DNA topoisomerase 4 subunit B from Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)

Seq: 647 a.a.

Struc: 208 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DNA/RNA chains

C-A-T-G-A-A-T 7 bases

A-G-T-C-A-T-T-C-A-T-G 11 bases

C-G-T-G-C-A-T 7 bases

G-A-C-T-A-T-G-C-A-C-G 11 bases

Enzyme reactions

• Enzyme class: Chains A, B, C, D: E.C.5.6.2.2 - Dna topoisomerase (ATP-hydrolyzing).