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PDBsum entry 4kpb

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protein ligands Protein-protein interface(s) links
Oxidoreductase PDB id
4kpb
Jmol
Contents
Protein chains
440 a.a.
Ligands
HEM ×2
Waters ×772
PDB id:
4kpb
Name: Oxidoreductase
Title: Crystal structure of cytochrome p450 bm-3 r47e mutant
Structure: Cytochrome p450 bm-3. Chain: a, b. Fragment: unp residues 1-471. Synonym: bifunctional p-450/NADPH-p450 reductase, cytochrom 3), cytochrome p450 102. Engineered: yes. Mutation: yes
Source: Bacillus megaterium. Organism_taxid: 1404. Gene: cyp102a1, cyp102. Expressed in: escherichia coli. Expression_system_taxid: 562
Resolution:
2.10Å     R-factor:   0.188     R-free:   0.232
Authors: K.Sadre-Bazzaz,J.Catalano,A.E.Mcdermott,L.Tong
Key ref: J.Catalano et al. (2013). Structural evidence: a single charged residue affects substrate binding in cytochrome P450 BM-3. Biochemistry, 52, 6807-6815. PubMed id: 23829560 DOI: 10.1021/bi4000645
Date:
13-May-13     Release date:   24-Jul-13    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P14779  (CPXB_BACME) -  Bifunctional P-450/NADPH-P450 reductase
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
1049 a.a.
440 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class 2: E.C.1.14.14.1  - Unspecific monooxygenase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O
RH
+ reduced flavoprotein
+ O(2)
= ROH
+ oxidized flavoprotein
+ H(2)O
      Cofactor: Heme-thiolate
   Enzyme class 3: E.C.1.6.2.4  - NADPH--hemoprotein reductase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein
NADPH
+ n oxidized hemoprotein
= NADP(+)
+ n reduced hemoprotein
      Cofactor: FAD; FMN
FAD
FMN
Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     oxidation-reduction process   1 term 
  Biochemical function     oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen     3 terms  

 

 
    reference    
 
 
DOI no: 10.1021/bi4000645 Biochemistry 52:6807-6815 (2013)
PubMed id: 23829560  
 
 
Structural evidence: a single charged residue affects substrate binding in cytochrome P450 BM-3.
J.Catalano, K.Sadre-Bazzaz, G.A.Amodeo, L.Tong, A.McDermott.
 
  ABSTRACT  
 
No abstract given.