PDBsum entry 4j4l

Protein binding/cytokine

PDB id: 4j4l

Contents

Protein chains

258 a.a.

155 a.a.

166 a.a.

Waters ×158

PDB id:

4j4l

Name:

Protein binding/cytokine

Title:

Modular evolution and design of the protein binding interface

Structure:

Internalin b,repeat modules,variable lymphocyte receptor b. Chain: a, b. Fragment: unp residues 43-100,unp residues 141-232. Engineered: yes. Other_details: chimera of residues 43-100 from internalin b, repeat modules, residues 141-232 from variable lymphocyte receptor b, and 8 his tags. Interleukin-6. Chain: c, d.

Source:

Listeria monocytogenes, synthetic construct, eptatretus burgeri. Inshore hagfish. Organism_taxid: 1639, 32630, 7764. Gene: vlrb. Expressed in: escherichia coli. Expression_system_taxid: 562. Homo sapiens. Human.

Resolution:

2.30Å R-factor: 0.205 R-free: 0.268

Authors:

H.K.Cheong,H.J.Kim

Key ref:

J.Lee et al. Modular evolution and design of the protein binding interface. To be published, .

Date:

07-Feb-13 Release date: 12-Feb-14

Protein chains

A4L9V2  (A4L9V2_LISMN) -  Internalin B from Listeria monocytogenes

Seq: 630 a.a.

Struc: 258 a.a.*

Protein chains

Q4G1L3  (Q4G1L3_EPTBU) -  Variable lymphocyte receptor B from Eptatretus burgeri

Seq: 305 a.a.

Struc: 258 a.a.*

Protein chain

P05231  (IL6_HUMAN) -  Interleukin-6 from Homo sapiens

Seq: 212 a.a.

Struc: 155 a.a.*

Protein chain

P05231  (IL6_HUMAN) -  Interleukin-6 from Homo sapiens

Seq: 212 a.a.

Struc: 166 a.a.

* PDB and UniProt seqs differ at 221 residue positions (black crosses)