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PDBsum entry 4h23

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protein ligands Protein-protein interface(s) links
Oxidoreductase PDB id
4h23
Jmol
Contents
Protein chains
454 a.a.
Ligands
HEM ×2
Waters ×24
PDB id:
4h23
Name: Oxidoreductase
Title: Cytochrome p411bm3-cis cyclopropanation catalyst
Structure: Cytochrome p450-bm3 variant p411bm3-cis. Chain: a, b. Fragment: heme domain (unp residues 1-464). Synonym: bifunctional p-450/NADPH-p450 reductase, cytochrom 3), cytochrome p450bm-3, cytochrome p450 102. Engineered: yes. Mutation: yes
Source: Bacillus megaterium. Organism_taxid: 1404. Gene: cyp102a1, cyp102. Expressed in: escherichia coli. Expression_system_taxid: 562
Resolution:
3.30Å     R-factor:   0.183     R-free:   0.265
Authors: P.S.Coelho,Z.J.Wang,M.E.Ener,S.A.Baril,A.Kannan,F.H.Arnold, E.M.Brustad
Key ref: P.S.Coelho et al. (2013). A serine-substituted P450 catalyzes highly efficient carbene transfer to olefins in vivo. Nat Chem Biol, 9, 485-487. PubMed id: 23792734 DOI: 10.1038/nchembio.1278
Date:
11-Sep-12     Release date:   26-Jun-13    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P14779  (CPXB_BACME) -  Bifunctional P-450/NADPH-P450 reductase
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
1049 a.a.
454 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 14 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class 2: E.C.1.14.14.1  - Unspecific monooxygenase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O
RH
+ reduced flavoprotein
+ O(2)
= ROH
+ oxidized flavoprotein
+ H(2)O
      Cofactor: Heme-thiolate
   Enzyme class 3: E.C.1.6.2.4  - NADPH--hemoprotein reductase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein
NADPH
+ n oxidized hemoprotein
= NADP(+)
+ n reduced hemoprotein
      Cofactor: FAD; FMN
FAD
FMN
Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     oxidation-reduction process   1 term 
  Biochemical function     oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen     3 terms  

 

 
    reference    
 
 
DOI no: 10.1038/nchembio.1278 Nat Chem Biol 9:485-487 (2013)
PubMed id: 23792734  
 
 
A serine-substituted P450 catalyzes highly efficient carbene transfer to olefins in vivo.
P.S.Coelho, Z.J.Wang, M.E.Ener, S.A.Baril, A.Kannan, F.H.Arnold, E.M.Brustad.
 
  ABSTRACT  
 
No abstract given.