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PDBsum entry 4gs0

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protein ligands Protein-protein interface(s) links
Hydrolase/transferase PDB id
4gs0
Jmol
Contents
Protein chains
279 a.a.
258 a.a.
Ligands
UNK-UNK-FTY-UNK
Waters ×287
PDB id:
4gs0
Name: Hydrolase/transferase
Title: Crystal structure of shp1 catalytic domain with jak1 activat peptide
Structure: Tyrosine-protein phosphatase non-receptor type 6. Chain: a, b. Fragment: phosphatase domain (unp residues 242-528). Synonym: hematopoietic cell protein-tyrosine phosphatase, p tyrosine phosphatase 1c, ptp-1c, protein-tyrosine phosphata sh-ptp1. Engineered: yes. Tyrosine-protein kinase jak1. Chain: c.
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: hcp, ptp1c, ptpn6, shp-1. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Organism_taxid: 9606
Resolution:
1.80Å     R-factor:   0.163     R-free:   0.195
Authors: N.L.Alicea-Velazquez,J.Jakoncic,T.J.Boggon
Key ref: N.L.Alicea-Velázquez et al. (2013). Structure-guided studies of the SHP-1/JAK1 interaction provide new insights into phosphatase catalytic domain substrate recognition. J Struct Biol, 181, 243-251. PubMed id: 23296072 DOI: 10.1016/j.jsb.2012.12.009
Date:
27-Aug-12     Release date:   19-Dec-12    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P23458  (JAK1_HUMAN) -  Tyrosine-protein kinase JAK1
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
1154 a.a.
279 a.a.*
Protein chain
Pfam   ArchSchema ?
P29350  (PTN6_HUMAN) -  Tyrosine-protein phosphatase non-receptor type 6
Seq:
Struc:
 
Seq:
Struc:
595 a.a.
279 a.a.
Protein chain
Pfam   ArchSchema ?
P29350  (PTN6_HUMAN) -  Tyrosine-protein phosphatase non-receptor type 6
Seq:
Struc:
 
Seq:
Struc:
595 a.a.
258 a.a.
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 238 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class 2: Chain A: E.C.2.7.10.2  - Non-specific protein-tyrosine kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP
+ [protein]-L-tyrosine
=
ADP
Bound ligand (Het Group name = FTY)
matches with 45.83% similarity
+ [protein]-L-tyrosine phosphate
   Enzyme class 3: Chains A, B: E.C.3.1.3.48  - Protein-tyrosine-phosphatase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate
Protein tyrosine phosphate
+ H(2)O
= protein tyrosine
+ phosphate
Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     dephosphorylation   2 terms 
  Biochemical function     phosphatase activity     2 terms  

 

 
    reference    
 
 
DOI no: 10.1016/j.jsb.2012.12.009 J Struct Biol 181:243-251 (2013)
PubMed id: 23296072  
 
 
Structure-guided studies of the SHP-1/JAK1 interaction provide new insights into phosphatase catalytic domain substrate recognition.
N.L.Alicea-Velázquez, J.Jakoncic, T.J.Boggon.
 
  ABSTRACT  
 
No abstract given.