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Eye lens protein
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PDB id
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4gcr
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Contents |
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* Residue conservation analysis
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Gene Ontology (GO) functional annotation
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Cellular component
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cytoplasm
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2 terms
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Biological process
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eye development
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4 terms
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Biochemical function
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structural constituent of eye lens
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1 term
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DOI no:
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Acta Crystallogr D Biol Crystallogr
49:223-233
(1993)
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PubMed id:
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Structure of the bovine eye lens protein gammaB(gammaII)-crystallin at 1.47 A.
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S.Najmudin,
V.Nalini,
H.P.Driessen,
C.Slingsby,
T.L.Blundell,
D.S.Moss,
P.F.Lindley.
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ABSTRACT
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The molecular structure of calf gammaB-crystallin (previously called gammaII), a
lens-specific protein, has been refined to a crystallographic R factor of 18.1%
for all reflection data, between 8.0 and 1.47 A, 25 959 hkl measured at 293 (1)
K. 230 water molecules have been defined by difference Fourier techniques and
included in a restrained least-squares refinement. Difference Fourier maps
clearly indicated the presence of multiple sites for the sulfur atoms of Cys 18
and Cys 22 which were therefore given coupled second-site occupancies during the
refinement. The sulfur atom in the major position of Cys 22 is in the reduced
state. Either of the Cys 18 sites can form a high-energy disulfide bridge with
the minor position of Cys 22. The position of the carboxy terminus and many
other surface side chains have been further defined including the RGD signal
peptide. The hydration of the backbone and the interdomain region has been
analysed. 27 water molecules make extensive contacts to a single protein
molecule and thus contribute to its stability.
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Selected figure(s)
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Figure 7.
Fig. 7. A stereoview of hydrogen-bonding pattern maintaining the
conformation of the RGD sequence which is on the surface of
the molecule. The Asp 61 forms an intramolecular ion pair with
Arg 36. The sheet structure is extended by a network of waer
molecules.
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Figure 8.
Fig. 8. A stereo picture of the water network shielding the exposed
ydrophobic side chain of Pro 82. Note water molecules 300
and 365 are too close (i.95 A) and are probably the same
fluctuating molecule. The electron density is contoured at 0.6
.m.s.
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The above figures are
reprinted
by permission from the IUCr:
Acta Crystallogr D Biol Crystallogr
(1993,
49,
223-233)
copyright 1993.
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Figures were
selected
by an automated process.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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K.Srivastava,
R.Gupta,
J.M.Chaves,
and
O.P.Srivastava
(2009).
Truncated human betaB1-crystallin shows altered structural properties and interaction with human betaA3-crystallin.
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Biochemistry, 48,
7179-7189.
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P.Garcia-Barreno,
M.C.Guisasola,
and
A.Suarez
(2005).
Fluorescent and compositional changes in crystallin supramolecular structures in pig lens during development.
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Comp Biochem Physiol B Biochem Mol Biol, 141,
179-185.
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I.Navizet,
F.Cailliez,
and
R.Lavery
(2004).
Probing protein mechanics: residue-level properties and their use in defining domains.
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Biophys J, 87,
1426-1435.
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G.D'Alessio
(2002).
The evolution of monomeric and oligomeric betagamma-type crystallins. Facts and hypotheses.
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Eur J Biochem, 269,
3122-3130.
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A.Pande,
J.Pande,
N.Asherie,
A.Lomakin,
O.Ogun,
J.A.King,
N.H.Lubsen,
D.Walton,
and
G.B.Benedek
(2000).
Molecular basis of a progressive juvenile-onset hereditary cataract.
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Proc Natl Acad Sci U S A, 97,
1993-1998.
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N.Li,
M.Erman,
W.Pangborn,
W.L.Duax,
C.M.Park,
J.Bruenn,
and
D.Ghosh
(1999).
Structure of Ustilago maydis killer toxin KP6 alpha-subunit. A multimeric assembly with a central pore.
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J Biol Chem, 274,
20425-20431.
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PDB code:
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S.Palme,
R.Jaenicke,
and
C.Slingsby
(1998).
X-ray structures of three interface mutants of gammaB-crystallin from bovine eye lens.
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Protein Sci, 7,
611-618.
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S.Palme,
C.Slingsby,
and
R.Jaenicke
(1997).
Mutational analysis of hydrophobic domain interactions in gamma B-crystallin from bovine eye lens.
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Protein Sci, 6,
1529-1536.
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J.Pande,
A.Lomakin,
B.Fine,
O.Ogun,
I.Sokolinski,
and
G.Benedek
(1995).
Oxidation of gamma II-crystallin solutions yields dimers with a high phase separation temperature.
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Proc Natl Acad Sci U S A, 92,
1067-1071.
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T.Zhao,
and
F.A.Bettelheim
(1995).
Enthalpy and entropy of hydration of bovine crystallins.
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J Biol Chem, 270,
24961-24964.
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S.Zarina,
C.Slingsby,
R.Jaenicke,
Z.H.Zaidi,
H.Driessen,
and
N.Srinivasan
(1994).
Three-dimensional model and quaternary structure of the human eye lens protein gamma S-crystallin based on beta- and gamma-crystallin X-ray coordinates and ultracentrifugation.
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Protein Sci, 3,
1840-1846.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
