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PDBsum entry 4fl4

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protein ligands metals Protein-protein interface(s) links
Protein binding PDB id
4fl4
Jmol
Contents
Protein chains
68 a.a.
165 a.a.
308 a.a.
Ligands
SO4 ×58
Metals
_CA ×16
Waters ×43
PDB id:
4fl4
Name: Protein binding
Title: Scaffoldin conformation and dynamics revealed by a ternary c from the clostridium thermocellum cellulosome
Structure: Glycoside hydrolase family 9. Chain: a, d, g, j. Fragment: type i dockerin (unp residues 584-649). Engineered: yes. Scaffolding dockerin binding protein a. Chain: b, e, h, k. Fragment: type ii cohesin (unp residues 27-200). Engineered: yes. Cellulosome anchoring protein cohesin region.
Source: Clostridium thermocellum. Organism_taxid: 1515. Gene: cther_2220. Expressed in: escherichia coli. Expression_system_taxid: 511693. Gene: sdba. Expression_system_taxid: 562. Gene: clothdraft_2952. Expression_system_taxid: 562
Resolution:
2.80Å     R-factor:   0.193     R-free:   0.236
Authors: M.A.Currie,J.J.Adams,F.Faucher,E.A.Bayer,Z.Jia,S.P.Smith,Mon Kingston Bacterial Structural Genomics Initiative (Bsgi)
Key ref: M.A.Currie et al. (2012). Scaffoldin conformation and dynamics revealed by a ternary complex from the Clostridium thermocellum cellulosome. J Biol Chem, 287, 26953-26961. PubMed id: 22707718
Date:
14-Jun-12     Release date:   27-Jun-12    
Supersedes: 3p0d
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
A3DDN1  (GUND_CLOTH) -  Endoglucanase D
Seq:
Struc:
 
Seq:
Struc:
649 a.a.
68 a.a.*
Protein chains
Pfam   ArchSchema ?
P71143  (P71143_CLOTM) -  Scaffolding dockerin binding protein A
Seq:
Struc:
 
Seq:
Struc:
631 a.a.
165 a.a.
Protein chains
Pfam   ArchSchema ?
Q46453  (Q46453_CLOTM) -  CipA protein (Fragment)
Seq:
Struc:
 
Seq:
Struc:
638 a.a.
308 a.a.*
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 3 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: Chains A, D, G, J: E.C.3.2.1.4  - Cellulase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endohydrolysis of 1,4-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     carbohydrate metabolic process   2 terms 
  Biochemical function     carbohydrate binding     2 terms  

 

 
J Biol Chem 287:26953-26961 (2012)
PubMed id: 22707718  
 
 
Scaffoldin conformation and dynamics revealed by a ternary complex from the Clostridium thermocellum cellulosome.
M.A.Currie, J.J.Adams, F.Faucher, E.A.Bayer, Z.Jia, S.P.Smith.
 
  ABSTRACT  
 
No abstract given.