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PDBsum entry 4fik

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protein ligands metals links
Lyase PDB id
4fik
Jmol
Contents
Protein chain
257 a.a.
Ligands
TH7
TH0 ×2
D2S
GOL
SO4
Metals
_ZN
Waters ×313
PDB id:
4fik
Name: Lyase
Title: Human carbonic anhydrase ii h64a complexed with thioxolone h products
Structure: Carbonic anhydrase 2. Chain: a. Synonym: carbonate dehydratase ii, carbonic anhydrasE C, ca carbonic anhydrase ii, ca-ii. Engineered: yes. Mutation: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: ca2. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
1.20Å     R-factor:   0.117     R-free:   0.137
Authors: K.H.Sippel,C.Genis,L.Govindasamy,M.Agbandje-Mckenna,B.C.Trip R.Mckenna
Key ref: K.H.Sippel et al. (2010). Synchrotron Radiation Provides a Plausible Explanation for the Generation of a Free Radical Adduct of Thioxolone in Mutant Carbonic Anhydrase II. J Phys Chem Lett, 1, 2898-2902. PubMed id: 20976122
Date:
08-Jun-12     Release date:   27-Jun-12    
Supersedes: 3iqk
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P00918  (CAH2_HUMAN) -  Carbonic anhydrase 2
Seq:
Struc:
260 a.a.
257 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.4.2.1.1  - Carbonate dehydratase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: H2CO3 = CO2 + H2O
H(2)CO(3)
= CO(2)
+ H(2)O
      Cofactor: Zn(2+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular space   11 terms 
  Biological process     angiotensin-mediated signaling pathway   21 terms 
  Biochemical function     protein binding     5 terms  

 

 
    Added reference    
 
 
J Phys Chem Lett 1:2898-2902 (2010)
PubMed id: 20976122  
 
 
Synchrotron Radiation Provides a Plausible Explanation for the Generation of a Free Radical Adduct of Thioxolone in Mutant Carbonic Anhydrase II.
K.H.Sippel, C.Genis, L.Govindasamy, M.Agbandje-McKenna, J.J.Kiddle, B.C.Tripp, R.McKenna.
 
  ABSTRACT  
 
No abstract given.