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PDBsum entry 4egj

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protein links
Ligase PDB id
4egj
Jmol
Contents
Protein chains
285 a.a.
246 a.a.
Waters ×358
PDB id:
4egj
Name: Ligase
Title: Crystal structure of d-alanine-d-alanine ligase from burkhol xenovorans
Structure: D-alanine--d-alanine ligase. Chain: a, b, c, d. Synonym: d-ala-d-ala ligase, d-alanylalanine synthetase. Engineered: yes
Source: Burkholderia xenovorans. Organism_taxid: 266265. Strain: lb400. Gene: bxeno_a3907, bxe_a0488, ddl. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Resolution:
2.30Å     R-factor:   0.225     R-free:   0.260
Authors: Ssgcid,Seattle Structural Genomics Center For Infectious Dis (Ssgcid)
Key ref: L.Baugh et al. (2013). Combining functional and structural genomics to sample the essential Burkholderia structome. PLoS One, 8, e53851. PubMed id: 23382856
Date:
31-Mar-12     Release date:   18-Jul-12    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q13TZ4  (DDL_BURXL) -  D-alanine--D-alanine ligase
Seq:
Struc:
313 a.a.
285 a.a.
Protein chain
Pfam   ArchSchema ?
Q13TZ4  (DDL_BURXL) -  D-alanine--D-alanine ligase
Seq:
Struc:
313 a.a.
246 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: Chains A, B, C, D: E.C.6.3.2.4  - D-alanine--D-alanine ligase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
Peptidoglycan Biosynthesis (Part 1)
      Reaction: ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine
ATP
+ 2 × D-alanine
= ADP
+ phosphate
+ D-alanyl-D-alanine
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cytoplasm   1 term 
  Biological process     cell wall organization   3 terms 
  Biochemical function     catalytic activity     8 terms  

 

 
    reference    
 
 
PLoS One 8:e53851 (2013)
PubMed id: 23382856  
 
 
Combining functional and structural genomics to sample the essential Burkholderia structome.
L.Baugh, L.A.Gallagher, R.Patrapuvich, M.C.Clifton, A.S.Gardberg, T.E.Edwards, B.Armour, D.W.Begley, S.H.Dieterich, D.M.Dranow, J.Abendroth, J.W.Fairman, D.Fox, B.L.Staker, I.Phan, A.Gillespie, R.Choi, S.Nakazawa-Hewitt, M.T.Nguyen, A.Napuli, L.Barrett, G.W.Buchko, R.Stacy, P.J.Myler, L.J.Stewart, C.Manoil, W.C.Van Voorhis.
 
  ABSTRACT  
 
No abstract given.