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PDBsum entry 4egc

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protein ligands metals Protein-protein interface(s) links
Transcription/hydrolase PDB id
4egc
Jmol
Contents
Protein chains
539 a.a.
255 a.a.
Ligands
MAL
Metals
_MG
Waters ×812
PDB id:
4egc
Name: Transcription/hydrolase
Title: Crystal structure of mbp-fused human six1 bound to human eya domain
Structure: Maltose-binding periplasmic protein, homeobox pro chimera. Chain: a. Fragment: see remark 999. Synonym: mbp, mmbp, maltodextrin-binding protein, sine ocul homeobox homolog 1. Engineered: yes. Mutation: yes. Eyes absent homolog 2.
Source: Escherichia coli, homo sapiens. Human. Organism_taxid: 562, 9606. Gene: b4034, jw3994, male, mbp, six1. Expressed in: escherichia coli. Expression_system_taxid: 562. Homo sapiens. Organism_taxid: 9606. Gene: eab1, eya2.
Resolution:
1.99Å     R-factor:   0.186     R-free:   0.224
Authors: R.Zhao,A.N.Patrick
Key ref: A.N.Patrick et al. (2013). Structure-function analyses of the human SIX1-EYA2 complex reveal insights into metastasis and BOR syndrome. Nat Struct Mol Biol, 20, 447-453. PubMed id: 23435380 DOI: 10.1038/nsmb.2505
Date:
30-Mar-12     Release date:   27-Feb-13    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P0AEX9  (MALE_ECOLI) -  Maltose-binding periplasmic protein
Seq:
Struc:
 
Seq:
Struc:
396 a.a.
539 a.a.*
Protein chain
Pfam   ArchSchema ?
Q15475  (SIX1_HUMAN) -  Homeobox protein SIX1
Seq:
Struc:
 
Seq:
Struc:
284 a.a.
539 a.a.
Protein chain
Pfam  
O00167  (EYA2_HUMAN) -  Eyes absent homolog 2
Seq:
Struc:
 
Seq:
Struc:
538 a.a.
255 a.a.
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 9 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: Chain B: E.C.3.1.3.48  - Protein-tyrosine-phosphatase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate
Protein tyrosine phosphate
+ H(2)O
= protein tyrosine
+ phosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     periplasmic space   5 terms 
  Biological process     multicellular organismal development   10 terms 
  Biochemical function     transporter activity     7 terms  

 

 
    reference    
 
 
DOI no: 10.1038/nsmb.2505 Nat Struct Mol Biol 20:447-453 (2013)
PubMed id: 23435380  
 
 
Structure-function analyses of the human SIX1-EYA2 complex reveal insights into metastasis and BOR syndrome.
A.N.Patrick, J.H.Cabrera, A.L.Smith, X.S.Chen, H.L.Ford, R.Zhao.
 
  ABSTRACT  
 
No abstract given.