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PDBsum entry 4efc

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protein ligands metals Protein-protein interface(s) links
Lyase PDB id
4efc
Jmol
Contents
Protein chains
452 a.a.
Ligands
AMP ×2
EDO ×2
Metals
_MG ×3
_NA
Waters ×711
PDB id:
4efc
Name: Lyase
Title: Crystal structure of adenylosuccinate lyase from trypanosoma tb427tmp.160.5560
Structure: Adenylosuccinate lyase. Chain: a, b. Engineered: yes
Source: Trypanosoma brucei brucei. Organism_taxid: 999953. Strain: 927/4 gutat10.1. Gene: tb09.160.5560. Expressed in: escherichia coli. Expression_system_taxid: 562
Resolution:
2.00Å     R-factor:   0.151     R-free:   0.191
Authors: A.K.Wernimont,P.Loppnau,K.T.Osman,C.H.Arrowsmith,A.M.Edwards C.Bountra,D.A.Robinson,P.G.Wyatt,I.H.Gilbert,A.H.Fairlamb,R Y.H.Lin,Structural Genomics Consortium (Sgc)
Key ref: A.K.Wernimont et al. Crystal structure of adenylosuccinate lyase from tryp brucei, Tb427tmp.160.5560. To be published, .
Date:
29-Mar-12     Release date:   13-Feb-13    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q38EJ2  (Q38EJ2_TRYB2) -  Adenylosuccinate lyase
Seq:
Struc:
471 a.a.
452 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.4.3.2.2  - Adenylosuccinate lyase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
Purine Biosynthesis (late stages)
      Reaction:
1. N6-(1,2-dicarboxyethyl)AMP = fumarate + AMP
2. (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D- ribosyl)imidazole-4-carboxamide
N(6)-(1,2-dicarboxyethyl)AMP
= fumarate
+
AMP
Bound ligand (Het Group name = AMP)
corresponds exactly
(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxamido)succinate
= fumarate
+
5-amino-1-(5-phospho-D- ribosyl)imidazole-4-carboxamide
Bound ligand (Het Group name = AMP)
matches with 87.50% similarity
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     'de novo' AMP biosynthetic process   5 terms 
  Biochemical function     catalytic activity     6 terms