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PDBsum entry 4ecu

Go to PDB code: 
protein dna_rna ligands metals links
Transferase/DNA PDB id
4ecu
Jmol
Contents
Protein chain
430 a.a.
DNA/RNA
Ligands
DTP
DPO
GOL ×2
Metals
_MG ×3
_CA
Waters ×302
PDB id:
4ecu
Name: Transferase/DNA
Title: Human DNA polymerase eta - DNA ternary complex: reaction in crystal at ph 7.0 for 200 sec
Structure: DNA polymerase eta. Chain: a. Fragment: catalytic core (unp residues 1-432). Synonym: rad30 homolog a, xeroderma pigmentosum variant typ engineered: yes. DNA (5'-d( Cp Ap Tp Tp Ap Tp Gp Ap Cp Gp Cp T)-3' chain: t. Engineered: yes. DNA (5'-d( Ap Gp Cp Gp Tp Cp Ap Tp A)-3').
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: polh, rad30, rad30a, xpv. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic: yes
Resolution:
1.95Å     R-factor:   0.175     R-free:   0.217
Authors: T.Nakamura,Y.Zhao,W.Yang
Key ref: T.Nakamura et al. (2012). Watching DNA polymerase η make a phosphodiester bond. Nature, 487, 196-201. PubMed id: 22785315
Date:
26-Mar-12     Release date:   11-Jul-12    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q9Y253  (POLH_HUMAN) -  DNA polymerase eta
Seq:
Struc:
 
Seq:
Struc:
713 a.a.
430 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.2.7.7.7  - DNA-directed Dna polymerase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Deoxynucleoside triphosphate
Bound ligand (Het Group name = DTP)
matches with 64.52% similarity
+ DNA(n)
=
diphosphate
Bound ligand (Het Group name = DPO)
corresponds exactly
+ DNA(n+1)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     DNA repair   1 term 
  Biochemical function     damaged DNA binding     2 terms  

 

 
    reference    
 
 
Nature 487:196-201 (2012)
PubMed id: 22785315  
 
 
Watching DNA polymerase η make a phosphodiester bond.
T.Nakamura, Y.Zhao, Y.Yamagata, Y.J.Hua, W.Yang.
 
  ABSTRACT  
 
No abstract given.