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PDBsum entry 4ecb

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protein Protein-protein interface(s) links
Transferase, protein binding PDB id
4ecb
Jmol
Contents
Protein chains
202 a.a.
Waters ×200
PDB id:
4ecb
Name: Transferase, protein binding
Title: Chimeric gst containing inserts of kininogen peptides
Structure: Chimeric protein between gshkt10 and domain 5 of 1. Chain: a, b. Fragment: unp residues 1-49, kinonogen 498-507, 50-228. Synonym: glutathione s-transferase class-mu 26 kda isozyme, sj26 antigen, sjgst/alpha-2-thiol proteinase inhibitor, fit factor, high molecular weight, kininogen, domain 5 of molecular weight kininogen. Engineered: yes
Source: Schistosoma japonicum, homo sapiens. Blood fluke, human. Organism_taxid: 6182, 9606. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Resolution:
2.20Å     R-factor:   0.202     R-free:   0.248
Authors: A.B.Amber,M.M.Sergei,P.Yi, Rita,R,Q.Xiaoping,P.-C.Marianne, C.M.William,Y.Vivien,R.M.Keith,A.K.Anton
Key ref: A.A.Bentley et al. (2012). Chimeric glutathione S-transferases containing inserts of kininogen peptides: potential novel protein therapeutics. J Biol Chem, 287, 22142-22150. PubMed id: 22577144 DOI: 10.1074/jbc.M112.372854
Date:
26-Mar-12     Release date:   16-May-12    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P08515  (GST26_SCHJA) -  Glutathione S-transferase class-mu 26 kDa isozyme
Seq:
Struc:
218 a.a.
202 a.a.
Protein chains
Pfam   ArchSchema ?
P01042  (KNG1_HUMAN) -  Kininogen-1
Seq:
Struc:
 
Seq:
Struc:
644 a.a.
202 a.a.*
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 177 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.2.5.1.18  - Glutathione transferase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: RX + glutathione = HX + R-S-glutathione
RX
+ glutathione
= HX
+ R-S-glutathione
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    reference    
 
 
DOI no: 10.1074/jbc.M112.372854 J Biol Chem 287:22142-22150 (2012)
PubMed id: 22577144  
 
 
Chimeric glutathione S-transferases containing inserts of kininogen peptides: potential novel protein therapeutics.
A.A.Bentley, S.M.Merkulov, Y.Peng, R.Rozmarynowycz, X.Qi, M.Pusztai-Carey, W.C.Merrick, V.C.Yee, K.R.McCrae, A.A.Komar.
 
  ABSTRACT  
 
No abstract given.