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PDBsum entry 4ec8

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protein Protein-protein interface(s) links
Transferase/protein binding PDB id
4ec8
Jmol
Contents
Protein chains
326 a.a.
252 a.a.
PDB id:
4ec8
Name: Transferase/protein binding
Title: Structure of full length cdk9 in complex with cyclint and dr
Structure: Cyclin-dependent kinase 9. Chain: a. Synonym: c-2k, cell division cycle 2-like protein kinase 4, division protein kinase 9, serine/threonine-protein kinase tat-associated kinase complex catalytic subunit. Engineered: yes. Cyclin-t1. Chain: b. Fragment: cyclin t, cyclin domain (unp residues 2-259).
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: cdk9, cdc2l4, tak. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: ccnt1. Expression_system_taxid: 7108
Resolution:
3.60Å     R-factor:   0.194     R-free:   0.230
Authors: Baumli S,Hole Aj,Endicott Ja
Key ref: S.Baumli et al. (2012). The CDK9 tail determines the reaction pathway of positive transcription elongation factor b. Structure, 20, 1788-1795. PubMed id: 22959624 DOI: 10.1016/j.str.2012.08.011
Date:
26-Mar-12     Release date:   12-Sep-12    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P50750  (CDK9_HUMAN) -  Cyclin-dependent kinase 9
Seq:
Struc:
372 a.a.
326 a.a.*
Protein chain
Pfam   ArchSchema ?
O60563  (CCNT1_HUMAN) -  Cyclin-T1
Seq:
Struc:
 
Seq:
Struc:
726 a.a.
252 a.a.*
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 4 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class 2: Chain A: E.C.2.7.11.22  - Cyclin-dependent kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + a protein = ADP + a phosphoprotein
ATP
+ protein
= ADP
+ phosphoprotein
   Enzyme class 3: Chain A: E.C.2.7.11.23  - [RNA-polymerase]-subunit kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
ATP
+ [DNA-directed RNA polymerase]
= ADP
+ [DNA-directed RNA polymerase] phosphate
Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     membrane   9 terms 
  Biological process     viral reproduction   21 terms 
  Biochemical function     transcription regulatory region DNA binding     15 terms  

 

 
    reference    
 
 
DOI no: 10.1016/j.str.2012.08.011 Structure 20:1788-1795 (2012)
PubMed id: 22959624  
 
 
The CDK9 tail determines the reaction pathway of positive transcription elongation factor b.
S.Baumli, A.J.Hole, L.Z.Wang, M.E.Noble, J.A.Endicott.
 
  ABSTRACT  
 
No abstract given.