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PDBsum entry 4duc

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protein ligands Protein-protein interface(s) links
Oxidoreductase PDB id
4duc
Jmol
Contents
Protein chains
456 a.a.
Ligands
HEM ×2
Waters ×568
PDB id:
4duc
Name: Oxidoreductase
Title: Cytochrome p450 bm3h-2g9 mri sensor, no ligand
Structure: Cytochrome p450 bm3 variant 2g9. Chain: a, b. Fragment: heme domain, residues 1-465. Synonym: bifunctional p-450/NADPH-p450 reductase, cytochrom 3), cytochrome p450bm-3, cytochrome p450 102, NADPH--cytoch reductase. Engineered: yes. Mutation: yes
Source: Bacillus megaterium. Organism_taxid: 1404. Gene: cyp102a1, cyp102. Expressed in: escherichia coli. Expression_system_taxid: 562
Resolution:
1.92Å     R-factor:   0.187     R-free:   0.226
Authors: E.M.Brustad,V.S.Lelyveld,C.D.Snow,N.Crook,F.M.Martinez,T.J.S A.Jasanoff,F.H.Arnold
Key ref: E.M.Brustad et al. (2012). Structure-guided directed evolution of highly selective p450-based magnetic resonance imaging sensors for dopamine and serotonin. J Mol Biol, 422, 245-262. PubMed id: 22659321 DOI: 10.1016/j.jmb.2012.05.029
Date:
21-Feb-12     Release date:   13-Jun-12    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P14779  (CPXB_BACME) -  Bifunctional P-450/NADPH-P450 reductase
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
1049 a.a.
456 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 4 residue positions (black crosses)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     oxidation-reduction process   1 term 
  Biochemical function     oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen     3 terms  

 

 
DOI no: 10.1016/j.jmb.2012.05.029 J Mol Biol 422:245-262 (2012)
PubMed id: 22659321  
 
 
Structure-guided directed evolution of highly selective p450-based magnetic resonance imaging sensors for dopamine and serotonin.
E.M.Brustad, V.S.Lelyveld, C.D.Snow, N.Crook, S.T.Jung, F.M.Martinez, T.J.Scholl, A.Jasanoff, F.H.Arnold.
 
  ABSTRACT  
 
No abstract given.