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PDBsum entry 4dql

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protein ligands links
Oxidoreductase PDB id
4dql
Jmol
Contents
Protein chains
378 a.a.
Ligands
FAD ×2
NAP ×2
SO4 ×2
1PE
PG4 ×3
Waters ×717
PDB id:
4dql
Name: Oxidoreductase
Title: Crystal structure of the fad binding domain of cytochrome p4 complex with NADP+
Structure: Bifunctional p-450/NADPH-p450 reductase. Chain: a, b. Fragment: cytochrome p450 bm3,unp residues 657-1049. Synonym: cytochrome p450(bm-3), cytochrome p450bm-3, cytoch 102, NADPH--cytochrome p450 reductase. Engineered: yes
Source: Bacillus megaterium. Organism_taxid: 1404. Gene: cyp102a1, cyp102. Expressed in: escherichia coli. Expression_system_taxid: 562
Resolution:
2.15Å     R-factor:   0.192     R-free:   0.226
Authors: M.G.Joyce,D.Leys
Key ref: M.G.Joyce et al. (2012). The crystal structure of the FAD/NADPH-binding domain of flavocytochrome P450 BM3. FEBS J, 279, 1694-1706. PubMed id: 22356131
Date:
16-Feb-12     Release date:   07-Mar-12    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P14779  (CPXB_BACME) -  Bifunctional P-450/NADPH-P450 reductase
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
1049 a.a.
378 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class 2: E.C.1.14.14.1  - Unspecific monooxygenase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O
RH
+ reduced flavoprotein
+ O(2)
= ROH
+ oxidized flavoprotein
+ H(2)O
      Cofactor: Heme-thiolate
   Enzyme class 3: E.C.1.6.2.4  - NADPH--hemoprotein reductase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein
NADPH
+ n oxidized hemoprotein
= NADP(+)
+ n reduced hemoprotein
      Cofactor: FAD; FMN
FAD
FMN
Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     oxidation-reduction process   1 term 
  Biochemical function     oxidoreductase activity     1 term  

 

 
    reference    
 
 
FEBS J 279:1694-1706 (2012)
PubMed id: 22356131  
 
 
The crystal structure of the FAD/NADPH-binding domain of flavocytochrome P450 BM3.
M.G.Joyce, I.S.Ekanem, O.Roitel, A.J.Dunford, R.Neeli, H.M.Girvan, G.J.Baker, R.A.Curtis, A.W.Munro, D.Leys.
 
  ABSTRACT  
 
No abstract given.