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PDBsum entry 4bag

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protein ligands metals Protein-protein interface(s) links
Hydrolase PDB id
4bag
Jmol
Contents
Protein chains
283 a.a.
Ligands
GOL
Metals
_CD ×8
Waters ×318
PDB id:
4bag
Name: Hydrolase
Title: Feruloyl esterase domain of xyny from clostridium thermocellum after exposure to 266nm uv laser
Structure: Endo-1,4-beta-xylanase y. Chain: a, b. Synonym: xylanase y, 1,4-beta-d-xylan xylanohydrolase y, xy engineered: yes
Source: Clostridium thermocellum. Organism_taxid: 1515. Expressed in: escherichia coli. Expression_system_taxid: 562
Resolution:
1.90Å     R-factor:   0.168     R-free:   0.187
Authors: P.J.B.Pereira,D.De Sanctis
Key ref: P.J.Pereira et al. (2013). In-house UV radiation-damage-induced phasing of selenomethionine-labeled protein structures. J Struct Biol, 181, 89-94. PubMed id: 23178456
Date:
14-Sep-12     Release date:   17-Apr-13    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P51584  (XYNY_CLOTM) -  Endo-1,4-beta-xylanase Y
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
1077 a.a.
283 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 2 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.3.2.1.8  - Endo-1,4-beta-xylanase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endohydrolysis of 1,4-beta-D-xylosidic linkages in xylans.

 

 
J Struct Biol 181:89-94 (2013)
PubMed id: 23178456  
 
 
In-house UV radiation-damage-induced phasing of selenomethionine-labeled protein structures.
P.J.Pereira, A.Royant, S.Panjikar, D.de Sanctis.
 
  ABSTRACT  
 
Selenomethionine labeling is the most common technique used in protein crystallography to derivatize recombinant proteins for experimental phasing using anomalous scattering at tunable synchrotron beamlines. Recently, it has been shown that UV radiation depletes electron density of selenium atoms of selenomethionine residues and that UV radiation-damage-induced phasing (equivalent to single isomorphous replacement) protocol can be applied to calculate experimental phases. Here we present the straightforward integration of a UV source with an in-house diffractometer. We show how this setup can extend the capabilities of a sealed tube X-ray generator and be used for experimental phasing of selenium-labeled proteins.