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PDBsum entry 4atq

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protein ligands Protein-protein interface(s) links
Transferase PDB id
4atq
Jmol
Contents
Protein chains
(+ 6 more) 442 a.a.
Ligands
PLP-ABU ×12
Waters ×546
PDB id:
4atq
Name: Transferase
Title: Gaba-transaminase a1r958 in complex with external aldimine p adduct
Structure: 4-aminobutyrate transaminase. Chain: a, b, c, d, e, f, g, h, i, j, k, l. Synonym: a1r958 transaminase. Engineered: yes. Other_details: covalent link between side chan of lys295 an aldehydic carbon of plp
Source: Arthrobacter aurescens. Organism_taxid: 290340. Strain: tc1. Expressed in: escherichia coli. Expression_system_taxid: 469008. Other_details: genomic DNA from atcc
Resolution:
2.75Å     R-factor:   0.204     R-free:   0.248
Authors: H.Bruce,A.N.Tuan,J.Mangas Sanchez,S.Hart,J.P.Turkenburg,G.Gr
Key ref: H.Bruce et al. (2012). Structures of a γ-aminobutyrate (GABA) transaminase from the s-triazine-degrading organism Arthrobacter aurescens TC1 in complex with PLP and with its external aldimine PLP-GABA adduct. Acta Crystallogr Sect F Struct Biol Cryst Commun, 68, 1175-1180. PubMed id: 23027742 DOI: 10.1107/S1744309112030023
Date:
09-May-12     Release date:   10-Oct-12    
PROCHECK
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 Headers
 References

Protein chains
No UniProt id for this chain
Struc: 442 a.a.
Key:    Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.2.6.1.19  - 4-aminobutyrate--2-oxoglutarate transaminase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: 4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate
4-aminobutanoate
Bound ligand (Het Group name = ABU)
corresponds exactly
+ 2-oxoglutarate
= succinate semialdehyde
+ L-glutamate
      Cofactor: Pyridoxal 5'-phosphate
Pyridoxal 5'-phosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     gamma-aminobutyric acid metabolic process   1 term 
  Biochemical function     catalytic activity     5 terms  

 

 
    reference    
 
 
DOI no: 10.1107/S1744309112030023 Acta Crystallogr Sect F Struct Biol Cryst Commun 68:1175-1180 (2012)
PubMed id: 23027742  
 
 
Structures of a γ-aminobutyrate (GABA) transaminase from the s-triazine-degrading organism Arthrobacter aurescens TC1 in complex with PLP and with its external aldimine PLP-GABA adduct.
H.Bruce, A.Nguyen Tuan, J.Mangas Sánchez, C.Leese, J.Hopwood, R.Hyde, S.Hart, J.P.Turkenburg, G.Grogan.
 
  ABSTRACT  
 
Two complex structures of the γ-aminobutyrate (GABA) transaminase A1R958 from Arthrobacter aurescens TC1 are presented. The first, determined to a resolution of 2.80 Å, features the internal aldimine formed by reaction between the ℇ-amino group of Lys295 and the cofactor pyridoxal phosphate (PLP); the second, determined to a resolution of 2.75 Å, features the external aldimine adduct formed between PLP and GABA in the first half-reaction. This is the first structure of a microbial GABA transaminase in complex with its natural external aldimine and reveals the molecular determinants of GABA binding in this enzyme.