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PDBsum entry 4abt

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protein dna_rna metals Protein-protein interface(s) links
Hydrolase/DNA PDB id
4abt
Jmol
Contents
Protein chains
286 a.a.
DNA/RNA
Metals
_CA ×3
Waters ×246
PDB id:
4abt
Name: Hydrolase/DNA
Title: Crystal structure of type iif restriction endonuclease ngomi cognate uncleaved DNA
Structure: Type-2 restriction enzyme ngomiv. Chain: a, b. Synonym: type iif restriction endonuclease ngomiv, r.Ngomiv endonuclease ngomiv, type ii restriction enzyme ngomiv. Engineered: yes. 5'-d( Tp Gp Cp Gp Cp Cp Gp Gp Cp Gp Cp)-3'. Chain: e, g
Source: Neisseria gonorrhoeae. Organism_taxid: 485. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Organism_taxid: 485
Resolution:
2.22Å     R-factor:   0.185     R-free:   0.234
Authors: E.N.Manakova,S.Grazulis,M.Zaremba,G.Tamulaitiene,D.Golovenko V.Siksnys
Key ref: E.N.Manakova et al. Structure of type iif restriction endonuclease ngomiv cognate uncleaved DNA. To be published, .
Date:
11-Dec-11     Release date:   28-Dec-11    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P31032  (T2M4_NEIGO) -  Type-2 restriction enzyme NgoMIV
Seq:
Struc:
286 a.a.
286 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.3.1.21.4  - Type Ii site-specific deoxyribonuclease.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
      Cofactor: Magnesium
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     nucleic acid phosphodiester bond hydrolysis   3 terms 
  Biochemical function     hydrolase activity     5 terms