PDBsum entry 4a0c

Cell cycle

PDB id: 4a0c

Contents

Protein chains

1155 a.a.

692 a.a.

89 a.a.

80 a.a.

Metals

_ZN ×6

PDB id:

4a0c

Name:

Cell cycle

Title:

Structure of the cand1-cul4b-rbx1 complex

Structure:

Cullin-associated nedd8-dissociated protein 1. Chain: a, b. Synonym: cullin-associated and neddylation-dissociated protein 1, tbp-interacting protein of 120 kda a, tbp-interacting protein 120a, p120 cand1. Engineered: yes. Cullin-4b. Chain: c, e. Synonym: cul-4b.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: trichoplusia ni. Expression_system_taxid: 7111. Expression_system_cell_line: high five. Mus musculus. House mouse. Organism_taxid: 10090.

Resolution:

3.80Å R-factor: 0.242 R-free: 0.319

Authors:

A.Scrima,E.S.Fischer,M.Faty,H.Gut,N.H.Thoma

Key ref:

E.S.Fischer et al. (2011). The molecular basis of CRL4DDB2/CSA ubiquitin ligase architecture, targeting, and activation. Cell, 147, 1024-1039. PubMed id: 22118460

Date:

08-Sep-11 Release date: 30-Nov-11

Protein chains

Q86VP6  (CAND1_HUMAN) -  Cullin-associated NEDD8-dissociated protein 1 from Homo sapiens

Seq: 1230 a.a.

Struc: 1155 a.a.*

Protein chains

Q13620  (CUL4B_HUMAN) -  Cullin-4B from Homo sapiens

Seq: 913 a.a.

Struc: 692 a.a.

Protein chain

P62878  (RBX1_MOUSE) -  E3 ubiquitin-protein ligase RBX1 from Mus musculus

Seq: 108 a.a.

Struc: 89 a.a.

Protein chain

P62878  (RBX1_MOUSE) -  E3 ubiquitin-protein ligase RBX1 from Mus musculus

Seq: 108 a.a.

Struc: 80 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: Chains D, F: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine
• Enzyme class 3: Chains D, F: E.C.2.3.2.32 - cullin-RING-type E3 NEDD8 transferase.
• Reaction: S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]- L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N₆-[NEDD8- protein]-yl-[cullin]-L-lysine

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Cell 147:1024-1039 (2011)

PubMed id: 22118460

The molecular basis of CRL4DDB2/CSA ubiquitin ligase architecture, targeting, and activation.

E.S.Fischer, A.Scrima, K.Böhm, S.Matsumoto, G.M.Lingaraju, M.Faty, T.Yasuda, S.Cavadini, M.Wakasugi, F.Hanaoka, S.Iwai, H.Gut, K.Sugasawa, N.H.Thomä.

ABSTRACT

No abstract given.