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PDBsum entry 4a4c

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protein ligands metals Protein-protein interface(s) links
Ligase/transferase PDB id
4a4c
Jmol
Contents
Protein chains
388 a.a.
145 a.a.
Ligands
SER-ASP-GLY-PTR-
THR-PRO-GLU-PRO-
ALA
Metals
_CA
_ZN ×2
Waters ×10
PDB id:
4a4c
Name: Ligase/transferase
Title: Structure of phosphotyr371-c-cbl-ubch5b-zap-70 complex
Structure: E3 ubiquitin-protein ligase cbl. Chain: a. Fragment: tkb domain, linker helix region, and ring domain, residues 47-435. Synonym: casitas b-lineage lymphoma proto-oncogene, proto-o c-cbl, ring finger protein 55, signal transduction protein c-cbl. Engineered: yes. Tyrosine-protein kinase zap-70.
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: gold. Synthetic: yes.
Resolution:
2.70Å     R-factor:   0.205     R-free:   0.267
Authors: H.Dou,L.Buetow,A.Hock,G.J.Sibbet,K.H.Vousden,D.T.Huang
Key ref: H.Dou et al. (2012). Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl. Nat Struct Mol Biol, 19, 184-192. PubMed id: 22266821
Date:
08-Oct-11     Release date:   25-Jan-12    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P22681  (CBL_HUMAN) -  E3 ubiquitin-protein ligase CBL
Seq:
Struc:
 
Seq:
Struc:
906 a.a.
388 a.a.
Protein chain
Pfam   ArchSchema ?
P62837  (UB2D2_HUMAN) -  Ubiquitin-conjugating enzyme E2 D2
Seq:
Struc:
147 a.a.
145 a.a.
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: Chain C: E.C.6.3.2.19  - Ubiquitin--protein ligase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine
ATP
+ ubiquitin
+
protein lysine
Bound ligand (Het Group name = PRO)
matches with 50.00% similarity
= AMP
+ diphosphate
+ protein N-ubiquityllysine
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     protein complex   6 terms 
  Biological process     regulation of signaling   15 terms 
  Biochemical function     signal transducer activity     10 terms  

 

 
    reference    
 
 
Nat Struct Mol Biol 19:184-192 (2012)
PubMed id: 22266821  
 
 
Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl.
H.Dou, L.Buetow, A.Hock, G.J.Sibbet, K.H.Vousden, D.T.Huang.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
22902369 H.Dou, L.Buetow, G.J.Sibbet, K.Cameron, and D.T.Huang (2012).
BIRC7-E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer.
  Nat Struct Mol Biol, 19, 876-883.
PDB code: 4auq
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