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PDBsum entry 4a49

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protein metals Protein-protein interface(s) links
Ligase PDB id
4a49
Jmol
Contents
Protein chains
77 a.a.
146 a.a.
Metals
_ZN ×2
__K
Waters ×132
PDB id:
4a49
Name: Ligase
Title: Structure of phosphotyr371-c-cbl-ubch5b complex
Structure: E3 ubiquitin-protein ligase cbl. Chain: a. Fragment: c-cbl residues 354-435. Synonym: c-cbl, casitas b-lineage lymphoma proto-oncogene, proto-oncogenE C-cbl, ring finger protein 55, signal trans protein cbl. Mutation: yes. Other_details: tyr371 is phosphorylated. Ubiquitin-conjugating enzyme e2 d2.
Source: Homo sapiens. Human. Organism_taxid: 9606. Organism_taxid: 9606
Resolution:
2.21Å     R-factor:   0.169     R-free:   0.205
Authors: H.Dou,L.Buetow,A.Hock,G.J.Sibbet,K.H.Vousden,D.T.Huang
Key ref: H.Dou et al. (2012). Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl. Nat Struct Mol Biol, 19, 184-192. PubMed id: 22266821
Date:
07-Oct-11     Release date:   25-Jan-12    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P22681  (CBL_HUMAN) -  E3 ubiquitin-protein ligase CBL
Seq:
Struc:
 
Seq:
Struc:
906 a.a.
77 a.a.*
Protein chain
Pfam   ArchSchema ?
P62837  (UB2D2_HUMAN) -  Ubiquitin-conjugating enzyme E2 D2
Seq:
Struc:
147 a.a.
146 a.a.
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: Chain B: E.C.6.3.2.19  - Ubiquitin--protein ligase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine
ATP
+ ubiquitin
+ protein lysine
= AMP
+ diphosphate
+ protein N-ubiquityllysine
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     protein complex   4 terms 
  Biological process     regulation of signaling   14 terms 
  Biochemical function     nucleotide binding     7 terms  

 

 
    reference    
 
 
Nat Struct Mol Biol 19:184-192 (2012)
PubMed id: 22266821  
 
 
Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl.
H.Dou, L.Buetow, A.Hock, G.J.Sibbet, K.H.Vousden, D.T.Huang.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
22902369 H.Dou, L.Buetow, G.J.Sibbet, K.Cameron, and D.T.Huang (2012).
BIRC7-E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer.
  Nat Struct Mol Biol, 19, 876-883.
PDB code: 4auq
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.