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PDBsum entry 4a07

Go to PDB code: 
protein ligands metals links
Transferase PDB id
4a07
Jmol
Contents
Protein chain
285 a.a.
Ligands
ATP
AZ7 ×2
Metals
_CL ×5
_MG
Waters ×209
PDB id:
4a07
Name: Transferase
Title: Human pdk1 kinase domain in complex with allosteric activato bound to the pif-pocket
Structure: 3-phosphoinositide-dependent protein kinase 1. Chain: a. Fragment: catalytic domain, residues 50-359. Synonym: hpdk1. Engineered: yes. Mutation: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9.
Resolution:
1.85Å     R-factor:   0.160     R-free:   0.199
Authors: J.O.Schulze,L.A.Lopez-Garcia,W.Froehner,H.Zhang,J.Navratil,V S.Zeuzem,P.M.Alzari,S.Neimanis,M.Engel,R.M.Biondi
Key ref: L.A.Lopez-Garcia et al. (2011). Allosteric regulation of protein kinase PKCĪ¶ by the N-terminal C1 domain and small compounds to the PIF-pocket. Chem Biol, 18, 1463-1473. PubMed id: 22118680 DOI: 10.1016/j.chembiol.2011.08.010
Date:
08-Sep-11     Release date:   07-Dec-11    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
O15530  (PDPK1_HUMAN) -  3-phosphoinositide-dependent protein kinase 1
Seq:
Struc:
 
Seq:
Struc:
556 a.a.
285 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 2 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.2.7.11.1  - Non-specific serine/threonine protein kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + a protein = ADP + a phosphoprotein
ATP
Bound ligand (Het Group name = ATP)
corresponds exactly
+ protein
= ADP
+ phosphoprotein
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     protein phosphorylation   1 term 
  Biochemical function     transferase activity, transferring phosphorus-containing groups     4 terms  

 

 
    reference    
 
 
DOI no: 10.1016/j.chembiol.2011.08.010 Chem Biol 18:1463-1473 (2011)
PubMed id: 22118680  
 
 
Allosteric regulation of protein kinase PKCĪ¶ by the N-terminal C1 domain and small compounds to the PIF-pocket.
L.A.Lopez-Garcia, J.O.Schulze, W.Fröhner, H.Zhang, E.Süss, N.Weber, J.Navratil, S.Amon, V.Hindie, S.Zeuzem, T.J.Jørgensen, P.M.Alzari, S.Neimanis, M.Engel, R.M.Biondi.
 
  ABSTRACT  
 
No abstract given.