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PDBsum entry 3zzk

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protein ligands links
Transferase PDB id
3zzk
Jmol
Contents
Protein chain
396 a.a.
Ligands
PMP
SO4 ×4
GOL ×5
Waters ×423
PDB id:
3zzk
Name: Transferase
Title: Structure of an engineered aspartate aminotransferase
Structure: Aspartate aminotransferase. Chain: a. Synonym: aspat, transaminase a. Engineered: yes. Mutation: yes
Source: Escherichia coli. Organism_taxid: 562. Strain: ty103. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
1.78Å     R-factor:   0.173     R-free:   0.198
Authors: F.J.Fernandez,D.Devries,E.Pena-Soler,M.Coll,P.Christen,H.Geh M.C.Vega
Key ref: F.J.Fernandez et al. (2012). Structure and mechanism of a cysteine sulfinate desulfinase engineered on the aspartate aminotransferase scaffold. Biochim Biophys Acta, 1824, 339-349. PubMed id: 22138634
Date:
01-Sep-11     Release date:   28-Dec-11    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P00509  (AAT_ECOLI) -  Aspartate aminotransferase
Seq:
Struc:
396 a.a.
396 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 3 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.2.6.1.1  - Aspartate transaminase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
L-aspartate
+ 2-oxoglutarate
=
oxaloacetate
Bound ligand (Het Group name = GOL)
matches with 50.00% similarity
+ L-glutamate
      Cofactor: Pyridoxal 5'-phosphate
Pyridoxal 5'-phosphate
Bound ligand (Het Group name = PMP) matches with 88.24% similarity
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cytoplasm   2 terms 
  Biological process     biosynthetic process   4 terms 
  Biochemical function     catalytic activity     8 terms  

 

 
    reference    
 
 
Biochim Biophys Acta 1824:339-349 (2012)
PubMed id: 22138634  
 
 
Structure and mechanism of a cysteine sulfinate desulfinase engineered on the aspartate aminotransferase scaffold.
F.J.Fernandez, D.de Vries, E.Peña-Soler, M.Coll, P.Christen, H.Gehring, M.C.Vega.
 
  ABSTRACT  
 
No abstract given.