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PDBsum entry 3zfs

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protein ligands metals links
Oxidoreductase PDB id
3zfs
Jmol
Contents
Protein chains
385 a.a.*
215 a.a.*
278 a.a.*
Ligands
SF4 ×4
F42
FAD
Metals
FE2
FCO
_NI
* C-alpha coords only
PDB id:
3zfs
Name: Oxidoreductase
Title: Cryo-em structure of the f420-reducing nife-hydrogenase from a methanogenic archaeon with bound substrate
Structure: F420-reducing hydrogenase, subunit alpha. Chain: a. Synonym: frha, coenzyme f420 hydrogenase alpha subunit. Other_details: frha contains a nife center. F420-reducing hydrogenase, subunit gamma. Chain: b. Synonym: frhg, coenzyme f420 hydrogenase gamma subunit. Other_details: frhg contains three 4fe4s clusters. F420-reducing hydrogenase, subunit beta.
Source: Methanothermobacter marburgensis. Organism_taxid: 145263. Strain: dsm 2133. Strain: dsm 2133
Authors: D.J.Mills,S.Vitt,M.Strauss,S.Shima,J.Vonck
Key ref: D.J.Mills et al. (2013). De novo modeling of the F420-reducing [NiFe]-hydrogenase from a methanogenic archaeon by cryo-electron microscopy. Elife, 2, e00218. PubMed id: 23483797 DOI: 10.7554/eLife.00218
Date:
12-Dec-12     Release date:   06-Mar-13    
 Headers
 References

Protein chain
Pfam   ArchSchema ?
D9PYF9  (D9PYF9_METTM) -  F420-reducing hydrogenase, subunit alpha
Seq:
Struc:
405 a.a.
385 a.a.
Protein chain
Pfam   ArchSchema ?
D9PYF7  (D9PYF7_METTM) -  F420-reducing hydrogenase, subunit gamma
Seq:
Struc:
275 a.a.
215 a.a.
Protein chain
Pfam   ArchSchema ?
D9PYF6  (D9PYF6_METTM) -  F420-reducing hydrogenase, subunit beta
Seq:
Struc:
281 a.a.
278 a.a.
Key:    PfamA domain  Secondary structure

 Enzyme reactions 
   Enzyme class: Chains A, B, C: E.C.1.12.98.1  - Coenzyme F420 hydrogenase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
Coenzyme F420 hydrogenase
      Reaction: H2 + oxidized coenzyme F420 = reduced coenzyme F420
H(2)
+ oxidized coenzyme F420
= reduced coenzyme F420
      Cofactor: FAD; Fe cation; Ni(2+)
FAD
Bound ligand (Het Group name = FAD) corresponds exactly
Fe cation
Ni(2+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     oxidation-reduction process   1 term 
  Biochemical function     oxidoreductase activity     9 terms  

 

 
    Added reference    
 
 
DOI no: 10.7554/eLife.00218 Elife 2:e00218 (2013)
PubMed id: 23483797  
 
 
De novo modeling of the F420-reducing [NiFe]-hydrogenase from a methanogenic archaeon by cryo-electron microscopy.
D.J.Mills, S.Vitt, M.Strauss, S.Shima, J.Vonck.
 
  ABSTRACT  
 
No abstract given.