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PDBsum entry 3wkt

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protein ligands Protein-protein interface(s) links
Oxidoreductase PDB id
3wkt
Jmol
Contents
Protein chains
602 a.a.
214 a.a.
Ligands
FAD ×2
FMN ×2
NAP ×2
HEM ×2
PDB id:
3wkt
Name: Oxidoreductase
Title: Complex structure of an open form of NADPH-cytochrome p450 r and heme oxygenase-1
Structure: NADPH-cytochrome p450 reductase. Chain: a, b. Synonym: cpr, p450r. Engineered: yes. Mutation: yes. Heme oxygenase 1. Chain: c, d. Synonym: ho-1, hsp32. Engineered: yes.
Source: Rattus norvegicus. Brown rat,rat,rats. Organism_taxid: 10116. Gene: por. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: hmox1.
Resolution:
4.30Å     R-factor:   0.224     R-free:   0.256
Authors: M.Sugishima,H.Sato,Y.Higashimoto,J.Harada,K.Wada,K.Fukuyama,
Key ref: M.Sugishima et al. Structural basis for the electron transfer from an op of NADPH-Cytochrome p450 oxidoreductase to heme oxyge. To be published, .
Date:
31-Oct-13     Release date:   29-Jan-14    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P00388  (NCPR_RAT) -  NADPH--cytochrome P450 reductase
Seq:
Struc:
 
Seq:
Struc:
678 a.a.
602 a.a.
Protein chains
Pfam   ArchSchema ?
P06762  (HMOX1_RAT) -  Heme oxygenase 1
Seq:
Struc:
289 a.a.
214 a.a.
Key:    PfamA domain  Secondary structure

 Enzyme reactions 
   Enzyme class 1: Chains A, B: E.C.1.6.2.4  - NADPH--hemoprotein reductase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein
NADPH
+ n oxidized hemoprotein
= NADP(+)
+ n reduced hemoprotein
      Cofactor: FAD; FMN
FAD
Bound ligand (Het Group name = FAD) corresponds exactly
FMN
   Enzyme class 2: Chains C, D: E.C.1.14.99.3  - Heme oxygenase (biliverdin-producing).
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Protoheme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O
Protoheme
Bound ligand (Het Group name = HEM)
matches with 95.45% similarity
+ 3 × AH(2)
+ 3 × O(2)
= n biliverdin
+ Fe(2+)
+ CO
+ 3 × A
+ 3 × H(2)O
Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     membrane   10 terms 
  Biological process     regulation of growth plate cartilage chondrocyte proliferation   63 terms 
  Biochemical function     electron carrier activity     20 terms