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PDBsum entry 3whq

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protein Protein-protein interface(s) links
Hydrolase, transferase PDB id
3whq
Jmol
Contents
Protein chains
360 a.a.
184 a.a.
Waters ×458
PDB id:
3whq
Name: Hydrolase, transferase
Title: Crystal structure of gamma-glutamyltranspeptidase from bacil subtilis (crystal soaked for 0 min. In acivicin soln. )
Structure: Gamma-glutamyltranspeptidase large chain. Chain: a. Synonym: l-subunit of gamma-glutamyltranspeptidase. Engineered: yes. Gamma-glutamyltranspeptidase small chain. Chain: b. Synonym: s-subunit of gamma-glutamyltranspeptidase. Engineered: yes
Source: Bacillus subtilis. Organism_taxid: 224308. Strain: 168. Gene: ggt, bsu18410. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
1.85Å     R-factor:   0.178     R-free:   0.214
Authors: K.Wada,K.Fukuyama
Key ref: T.Ida et al. (2014). Structure of bacillus subtilis gamma-Glutamyl-Transpe in complex with acivicin: diversity of the binding mo classical and electrophilic active-Site-Directed glut analogue. Acta crystallogr.,Sect.D, 70, 607. DOI: 10.1107/S1399004713031222
Date:
30-Aug-13     Release date:   19-Feb-14    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P54422  (GGT_BACSU) -  Gamma-glutamyltranspeptidase
Seq:
Struc:
 
Seq:
Struc:
587 a.a.
360 a.a.
Protein chain
Pfam   ArchSchema ?
P54422  (GGT_BACSU) -  Gamma-glutamyltranspeptidase
Seq:
Struc:
 
Seq:
Struc:
587 a.a.
184 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class 2: Chains A, B: E.C.2.3.2.2  - Gamma-glutamyltransferase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: A (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
(5-L-glutamyl)-peptide
+ amino acid
= peptide
+ 5-L-glutamyl amino acid
   Enzyme class 3: Chains A, B: E.C.3.4.19.13  - Glutathione hydrolase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Glutathione + H2O = L-cysteinylglycine + L-glutamate
Glutathione
+ H(2)O
= L-cysteinylglycine
+ L-glutamate
Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     glutathione metabolic process   1 term 
  Biochemical function     gamma-glutamyltransferase activity     1 term