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PDBsum entry 3vpf

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protein ligands Protein-protein interface(s) links
Oxidoreductase PDB id
3vpf
Jmol
Contents
Protein chains
(+ 0 more) 306 a.a.
Ligands
PYR ×3
SO4 ×9
Waters ×70
PDB id:
3vpf
Name: Oxidoreductase
Title: Complex structure of lactobacillus casei lactate dehydrogena mutant with pyruvate
Structure: L-lactate dehydrogenase. Chain: a, b, c, d, e, f. Synonym: l-ldh. Engineered: yes. Mutation: yes
Source: Lactobacillus casei. Organism_taxid: 1582. Gene: ldh. Expressed in: escherichia coli. Expression_system_taxid: 562
Resolution:
2.79Å     R-factor:   0.186     R-free:   0.259
Authors: K.Arai,A.Miyanaga,H.Uchikoba,S.Fushinobu,H.Taguchi
Key ref: K.Arai et al. Crystal structure of penta mutant of l-Lactate dehydr from lactobacillus casei. To be published, .
Date:
01-Mar-12     Release date:   06-Mar-13    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P00343  (LDH_LACCA) -  L-lactate dehydrogenase
Seq:
Struc:
326 a.a.
306 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 5 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.1.1.1.27  - L-lactate dehydrogenase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: (S)-lactate + NAD+ = pyruvate + NADH
(S)-lactate
Bound ligand (Het Group name = PYR)
corresponds exactly
+ NAD(+)
= pyruvate
+ NADH
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cytoplasm   1 term 
  Biological process     oxidation-reduction process   4 terms 
  Biochemical function     catalytic activity     4 terms