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PDBsum entry 3vht

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protein metals Protein-protein interface(s) links
Fluorescent protein/protein binding PDB id
3vht
Jmol
Contents
Protein chains
229 a.a.
260 a.a.
76 a.a.
Metals
_ZN
Waters ×133
PDB id:
3vht
Name: Fluorescent protein/protein binding
Title: Crystal structure of gfp-wrnip1 ubz domain fusion protein in with ubiquitin
Structure: Green fluorescent protein. Chain: a. Fragment: gfp domain. Engineered: yes. Green fluorescent protein, atpase wrnip1. Chain: b. Fragment: gfp-wrnip1 ubz domain. Synonym: werner helicase-interacting protein 1. Engineered: yes.
Source: Aequorea victoria. Jellyfish. Organism_taxid: 6100. Gene: gfp. Expressed in: escherichia coli. Expression_system_taxid: 562. Aequorea victoria, homo sapiens. Jellyfish, human. Organism_taxid: 6100, 9606.
Resolution:
2.40Å     R-factor:   0.194     R-free:   0.238
Authors: N.Suzuki,S.Wakatsuki,M.Kawasaki
Key ref: N.Suzuki et al. Structural analysis of the ubiquitin-Binding zinc fin human wrnip1in complex with ubiquitin.. To be published, .
Date:
06-Sep-11     Release date:   10-Oct-12    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P42212  (GFP_AEQVI) -  Green fluorescent protein
Seq:
Struc:
238 a.a.
229 a.a.*
Protein chain
Pfam   ArchSchema ?
Q96S55  (WRIP1_HUMAN) -  ATPase WRNIP1
Seq:
Struc:
 
Seq:
Struc:
665 a.a.
260 a.a.*
Protein chain
Pfam   ArchSchema ?
E9Q9J0  (E9Q9J0_MOUSE) -  Ubiquitin-60S ribosomal protein L40 (Fragment)
Seq:
Struc:
96 a.a.
76 a.a.
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 14 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: Chain B: E.C.3.6.1.3  - Adenosinetriphosphatase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + H2O = ADP + phosphate
ATP
+ H(2)O
= ADP
+ phosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cellular_component   1 term 
  Biological process     generation of precursor metabolites and energy   4 terms 
  Biochemical function     molecular_function     2 terms