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PDBsum entry 3vad

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protein ligands metals links
Transferase/transferase inhibitor PDB id
3vad
Jmol
Contents
Protein chain
310 a.a.
Ligands
ADP
0F1-0F1
Metals
_MG
__K
Waters ×2
PDB id:
3vad
Name: Transferase/transferase inhibitor
Title: Crystal structure of i170f mutant branched-chain alpha-ketoa dehydrogenase kinase in complex with 3,6-dichlorobenzo[b]th carboxylic acid
Structure: [3-methyl-2-oxobutanoate dehydrogenase [lipoamide mitochondrial. Chain: a. Synonym: branched-chain alpha-ketoacid dehydrogenase kinase kinase, bckdhkin. Engineered: yes. Mutation: yes
Source: Rattus norvegicus. Rat. Organism_taxid: 10116. Gene: bckdk. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
2.60Å     R-factor:   0.229     R-free:   0.276
Authors: K.Ahmed,W.J.Gui,S.C.Tso,J.L.Chuang,R.M Wynn,D.T.Chuang
Key ref: K.Ahmed et al. Crystal structure of I170f mutant branched-Chain alpha-Ketoacid dehydrogenase kinase in complex with 3,6-Dichlorobenzo[b]thiophene-2-Carboxylic acid. To be published, .
Date:
29-Dec-11     Release date:   16-Jan-13    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q00972  (BCKD_RAT) -  [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial
Seq:
Struc:
412 a.a.
310 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.2.7.11.4  - [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] = ADP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] phosphate
ATP
+ [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)]
=
ADP
Bound ligand (Het Group name = ADP)
corresponds exactly
+ [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] phosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     mitochondrion   3 terms 
  Biological process     phosphorylation   3 terms 
  Biochemical function     nucleotide binding     8 terms