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PDBsum entry 3v3f

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protein metals links
Lyase PDB id
3v3f
Jmol
Contents
Protein chain
257 a.a.
Metals
_CL
_ZN
Waters ×142
PDB id:
3v3f
Name: Lyase
Title: Kinetic and structural studies of thermostabilized mutants o
Structure: Carbonic anhydrase 2. Chain: a. Synonym: carbonate dehydratase ii, carbonic anhydrasE C, ca carbonic anhydrase ii, ca-ii. Engineered: yes. Mutation: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: ca2. Expressed in: escherichia coli. Expression_system_taxid: 562
Resolution:
2.00Å     R-factor:   0.191     R-free:   0.256
Authors: C.D.Boone,S.Z.Fisher,R.Mckenna
Key ref: Z.Fisher et al. (2012). Kinetic and structural characterization of thermostabilized mutants of human carbonic anhydrase II. Protein Eng Des Sel, 25, 347-355. PubMed id: 22691706
Date:
13-Dec-11     Release date:   20-Jun-12    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P00918  (CAH2_HUMAN) -  Carbonic anhydrase 2
Seq:
Struc:
260 a.a.
257 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 4 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.4.2.1.1  - Carbonate dehydratase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: H2CO3 = CO2 + H2O
H(2)CO(3)
= CO(2)
+ H(2)O
      Cofactor: Zinc
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular space   10 terms 
  Biological process     angiotensin-mediated signaling pathway   20 terms 
  Biochemical function     protein binding     5 terms  

 

 
    Added reference    
 
 
Protein Eng Des Sel 25:347-355 (2012)
PubMed id: 22691706  
 
 
Kinetic and structural characterization of thermostabilized mutants of human carbonic anhydrase II.
Z.Fisher, C.D.Boone, S.M.Biswas, B.Venkatakrishnan, M.Aggarwal, C.Tu, M.Agbandje-McKenna, D.Silverman, R.McKenna.
 
  ABSTRACT  
 
No abstract given.