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PDBsum entry 3usv

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protein links
Hydrolase PDB id
3usv
Jmol
Contents
Protein chains
308 a.a.
PDB id:
3usv
Name: Hydrolase
Title: Structure of the precursor of a thermostable variant of papa a resolution from a crystal soaked at ph 4
Structure: Papain. Chain: a, c. Fragment: unp residues 27-345. Synonym: papaya proteinase i, ppi. Engineered: yes. Mutation: yes
Source: Carica papaya. Mamon. Organism_taxid: 3649. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
3.80Å     R-factor:   0.358     R-free:   0.408
Authors: S.Roy,D.Choudhury,S.Biswas,J.K.Dattagupta
Key ref: S.Roy et al. Crystallographic analysis of pro-Papain variant eluci the structural basis of the step-Wise activation mech of the zymogen. To be published, .
Date:
24-Nov-11     Release date:   28-Nov-12    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P00784  (PAPA1_CARPA) -  Papain
Seq:
Struc:
345 a.a.
308 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 4 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.3.4.22.2  - Papain.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Hydrolysis of proteins with broad specificity for peptide bonds, with preference for a residue bearing a large hydrophobic sidechain at the P2 position. Does not accept Val at P1'.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     proteolysis   1 term 
  Biochemical function     hydrolase activity     3 terms