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PDBsum entry 3uip

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protein Protein-protein interface(s) links
Ligase/isomerase/protein binding PDB id
3uip
Jmol
Contents
Protein chains
157 a.a.
78 a.a.
156 a.a.
61 a.a.
Waters ×343
PDB id:
3uip
Name: Ligase/isomerase/protein binding
Title: Complex between human rangap1-sumo1, ubc9 and the ir1 domain ranbp2 containing ir2 motif ii
Structure: Sumo-conjugating enzyme ubc9. Chain: a. Synonym: sumo-protein ligase, ubiquitin carrier protein 9, carrier protein i, ubiquitin-conjugating enzyme e2 i, ubiqu protein ligase i, p18. Engineered: yes. Small ubiquitin-related modifier 1. Chain: b. Fragment: unp residues 18-97.
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: ubc9, ubce9, ube2i. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: ok/sw-cl.43, smt3c, smt3h3, sumo1, ubl1. Gene: kiaa1835, rangap1, sd. Gene: nup358, ranbp2.
Resolution:
2.29Å     R-factor:   0.194     R-free:   0.232
Authors: J.R.Gareau,D.Reverter,C.D.Lima
Key ref: J.R.Gareau et al. (2012). Determinants of small ubiquitin-like modifier 1 (SUMO1) protein specificity, E3 ligase, and SUMO-RanGAP1 binding activities of nucleoporin RanBP2. J Biol Chem, 287, 4740-4751. PubMed id: 22194619 DOI: 10.1074/jbc.M111.321141
Date:
05-Nov-11     Release date:   28-Dec-11    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P63279  (UBC9_HUMAN) -  SUMO-conjugating enzyme UBC9
Seq:
Struc:
158 a.a.
157 a.a.*
Protein chain
Pfam   ArchSchema ?
P63165  (SUMO1_HUMAN) -  Small ubiquitin-related modifier 1
Seq:
Struc:
101 a.a.
78 a.a.
Protein chain
Pfam   ArchSchema ?
P46060  (RAGP1_HUMAN) -  Ran GTPase-activating protein 1
Seq:
Struc:
 
Seq:
Struc:
587 a.a.
156 a.a.
Protein chain
Pfam   ArchSchema ?
P49792  (RBP2_HUMAN) -  E3 SUMO-protein ligase RanBP2
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
3224 a.a.
61 a.a.*
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 9 residue positions (black crosses)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     synapse   9 terms 
  Biological process     viral reproduction   18 terms 
  Biochemical function     nucleotide binding     15 terms  

 

 
DOI no: 10.1074/jbc.M111.321141 J Biol Chem 287:4740-4751 (2012)
PubMed id: 22194619  
 
 
Determinants of small ubiquitin-like modifier 1 (SUMO1) protein specificity, E3 ligase, and SUMO-RanGAP1 binding activities of nucleoporin RanBP2.
J.R.Gareau, D.Reverter, C.D.Lima.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
22842904 A.Plechanovová, E.G.Jaffray, M.H.Tatham, J.H.Naismith, and R.T.Hay (2012).
Structure of a RING E3 ligase and ubiquitin-loaded E2 primed for catalysis.
  Nature, 489, 115-120.
PDB code: 4ap4
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.