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PDBsum entry 3ufx

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protein ligands metals Protein-protein interface(s) links
Ligase PDB id
3ufx
Jmol
Contents
Protein chains
288 a.a.
378 a.a.
Ligands
GDP ×4
Metals
_MN ×4
Waters ×735
PDB id:
3ufx
Name: Ligase
Title: Thermus aquaticus succinyl-coa synthetase in complex with gd
Structure: Succinyl-coa synthetase alpha subunit. Chain: a, d, f, h. Synonym: succinyl-coa ligase alpha subunit. Engineered: yes. Succinyl-coa synthetase beta subunit. Chain: b, e, g, i. Synonym: succinyl-coa ligase beta subunit. Engineered: yes
Source: Thermus aquaticus. Organism_taxid: 271. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562
Resolution:
2.35Å     R-factor:   0.206     R-free:   0.245
Authors: M.E.Fraser
Key ref: M.A.Joyce et al. (2012). Biochemical and structural characterization of the GTP-preferring succinyl-CoA synthetase from Thermus aquaticus. Acta Crystallogr D Biol Crystallogr, 68, 751-762. PubMed id: 22751660 DOI: 10.1107/S0907444912010852
Date:
01-Nov-11     Release date:   27-Jun-12    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P09143  (SUCD_THETH) -  Succinyl-CoA ligase [ADP-forming] subunit alpha
Seq:
Struc:
288 a.a.
288 a.a.*
Protein chains
Pfam   ArchSchema ?
P25126  (SUCC_THETH) -  Succinyl-CoA ligase [ADP-forming] subunit beta
Seq:
Struc:
378 a.a.
378 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 6 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: Chains A, B, D, E, F, G, H, I: E.C.6.2.1.5  - Succinate--CoA ligase (ADP-forming).
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + succinate + CoA = ADP + phosphate + succinyl-CoA
ATP
+ succinate
+ CoA
=
ADP
Bound ligand (Het Group name = GDP)
matches with 96.43% similarity
+ phosphate
+ succinyl-CoA
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     metabolic process   2 terms 
  Biochemical function     catalytic activity     10 terms  

 

 
    reference    
 
 
DOI no: 10.1107/S0907444912010852 Acta Crystallogr D Biol Crystallogr 68:751-762 (2012)
PubMed id: 22751660  
 
 
Biochemical and structural characterization of the GTP-preferring succinyl-CoA synthetase from Thermus aquaticus.
M.A.Joyce, K.Hayakawa, W.T.Wolodko, M.E.Fraser.
 
  ABSTRACT  
 
No abstract given.