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PDBsum entry 3u6q

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protein dna_rna metals links
Hydrolase/DNA PDB id
3u6q
Jmol
Contents
Protein chain
252 a.a.
DNA/RNA
Metals
_ZN
Waters ×150
PDB id:
3u6q
Name: Hydrolase/DNA
Title: Mutm set 2 apgo
Structure: Formamidopyrimidine-DNA glycosylase. Chain: a. Engineered: yes. Mutation: yes. DNA (5'-d( Ap Gp Gp Tp Ap Gp Ap Cp Cp Tp Gp Gp Ap 3'). Chain: b. Engineered: yes. DNA (5'-d( Tp Gp Cp Gp Tp Cp Cp Ap (8Og)p Gp Tp (
Source: Geobacillus stearothermophilus. Organism_taxid: 1422. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: synthetic DNA. Other_details: synthetic DNA
Resolution:
1.98Å     R-factor:   0.203     R-free:   0.233
Authors: R.J.Sung,M.Zhang,Y.Qi,G.L.Verdine
Key ref: R.J.Sung et al. (2012). Sequence-dependent structural variation in DNA undergoing intrahelical inspection by the DNA glycosylase MutM. J Biol Chem, 287, 18044-18054. PubMed id: 22465958 DOI: 10.1074/jbc.M111.313635
Date:
12-Oct-11     Release date:   25-Apr-12    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P84131  (P84131_GEOSE) -  Formamidopyrimidine-DNA glycosylase
Seq:
Struc:
274 a.a.
252 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.3.2.2.23  - DNA-formamidopyrimidine glycosylase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Hydrolysis of DNA containing ring-opened N(7)-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimide.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     metabolic process   6 terms 
  Biochemical function     catalytic activity     12 terms  

 

 
DOI no: 10.1074/jbc.M111.313635 J Biol Chem 287:18044-18054 (2012)
PubMed id: 22465958  
 
 
Sequence-dependent structural variation in DNA undergoing intrahelical inspection by the DNA glycosylase MutM.
R.J.Sung, M.Zhang, Y.Qi, G.L.Verdine.
 
  ABSTRACT  
 
No abstract given.