PDBsum entry 3tux

Transferase

PDB id: 3tux

Contents

Protein chain

336 a.a.

Ligands

ATP

SO4

GOL

EDO ×2

Metals

_MN

Waters ×311

PDB id:

3tux

Name:

Transferase

Title:

Crystal structure of rtca.Atp.Mn ternary complex

Structure:

RNA 3'-terminal phosphate cyclase. Chain: a. Synonym: RNA cyclase, RNA-3'-phosphate cyclase. Engineered: yes. Mutation: yes

Source:

Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: b4475, jw5688, rtca, yhgj, yhgk. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.85Å R-factor: 0.189 R-free: 0.252

Authors:

A.K.Chakravarty,P.Smith,S.Shuman

Key ref:

A.K.Chakravarty et al. (2011). Structures of RNA 3'-phosphate cyclase bound to ATP reveal the mechanism of nucleotidyl transfer and metal-assisted catalysis. Proc Natl Acad Sci U S A, 108, 21034-21039. PubMed id: 22167800

Date:

19-Sep-11 Release date: 28-Dec-11

Protein chain

P46849  (RTCA_ECOLI) -  RNA 3'-terminal phosphate cyclase from Escherichia coli (strain K12)

Seq: 338 a.a.

Struc: 336 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.6.5.1.4 - Rna 3'-terminal-phosphate cyclase (ATP).
• Reaction: a 3'-end 3'-phospho-ribonucleotide-RNA + ATP = a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + AMP + diphosphate

3'-end 3'-phospho-ribonucleotide-RNA + ATP (Bound ligand (Het Group name = ATP) corresponds exactly) = 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + AMP + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Proc Natl Acad Sci U S A 108:21034-21039 (2011)

PubMed id: 22167800

Structures of RNA 3'-phosphate cyclase bound to ATP reveal the mechanism of nucleotidyl transfer and metal-assisted catalysis.

A.K.Chakravarty, P.Smith, S.Shuman.

ABSTRACT

No abstract given.