PDBsum entry 3tpy

Hydrolase

PDB id: 3tpy

Contents

Protein chain

113 a.a.

Ligands

TRS

DUP-UMP

Metals

_MG

Waters ×131

PDB id:

3tpy

Name:

Hydrolase

Title:

Crystal structure of m-pmv dutpase with a mixed population of substrate (dupnpp) and post-inversion product (dump) in the active sites

Structure:

Deoxyuridine 5'-triphosphate nucleotido hydrolase. Chain: a. Fragment: dutpase (catalytic) domain, unp residues 608-759. Engineered: yes. Mutation: yes

Source:

Mason-pfizer monkey virus. Mpmv. Organism_taxid: 11855. Gene: gag-pro. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.75Å R-factor: 0.154 R-free: 0.186

Authors:

O.Barabas,V.Nemeth,B.G.Vertessy

Key ref:

O.Barabas et al. Structural snapshots of enzyme-Catalysed phosphate es hydrolysis directly visualize in-Line attack and inve. To be published, .

Date:

08-Sep-11 Release date: 12-Oct-11

Protein chain

P07570  (PRO_MPMV) -  Gag-Pro polyprotein from Mason-Pfizer monkey virus

Seq: 911 a.a.

Struc: 113 a.a.

Enzyme reactions

• Enzyme class: E.C.3.6.1.23 - dUTP diphosphatase.
• Reaction: dUTP + H2O = dUMP + diphosphate + H⁺

dUTP + H2O = dUMP + diphosphate (Bound ligand (Het Group name = UMP) corresponds exactly) + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site