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PDBsum entry 3tni

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protein Protein-protein interface(s) links
Transferase/transferase inhibitor PDB id
3tni
Jmol
Contents
Protein chains
298 a.a.
252 a.a.
PDB id:
3tni
Name: Transferase/transferase inhibitor
Title: Structure of cdk9/cyclin t f241l
Structure: Cyclin-dependent kinase 9. Chain: a. Fragment: kinase domain, unp residues 2-330. Synonym: c-2k, cell division cycle 2-like protein kinase 4, division protein kinase 9, serine/threonine-protein kinase engineered: yes. Cyclin-t1. Chain: b. Fragment: cyclin boxes, unp residues 1-259.
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: cdc2l4, cdk9. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9. Gene: ccnt1.
Resolution:
3.23Å     R-factor:   0.166     R-free:   0.210
Authors: S.Baumli,A.J.Hole,J.A.Endicott
Key ref: S.Baumli et al. (2012). The CDK9 C-helix exhibits conformational plasticity that may explain the selectivity of CAN508. ACS Chem Biol, 7, 811-816. PubMed id: 22292676
Date:
01-Sep-11     Release date:   15-Feb-12    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P50750  (CDK9_HUMAN) -  Cyclin-dependent kinase 9
Seq:
Struc:
372 a.a.
298 a.a.*
Protein chain
Pfam   ArchSchema ?
O60563  (CCNT1_HUMAN) -  Cyclin-T1
Seq:
Struc:
 
Seq:
Struc:
726 a.a.
252 a.a.*
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 2 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class 2: Chain A: E.C.2.7.11.22  - Cyclin-dependent kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + a protein = ADP + a phosphoprotein
ATP
+ protein
= ADP
+ phosphoprotein
   Enzyme class 3: Chain A: E.C.2.7.11.23  - [RNA-polymerase]-subunit kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
ATP
+ [DNA-directed RNA polymerase]
= ADP
+ [DNA-directed RNA polymerase] phosphate
Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     transcription, DNA-dependent   4 terms 
  Biochemical function     transferase activity, transferring phosphorus-containing groups     5 terms  

 

 
    reference    
 
 
ACS Chem Biol 7:811-816 (2012)
PubMed id: 22292676  
 
 
The CDK9 C-helix exhibits conformational plasticity that may explain the selectivity of CAN508.
S.Baumli, A.J.Hole, M.E.Noble, J.A.Endicott.
 
  ABSTRACT  
 
No abstract given.