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PDBsum entry 3tg0

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protein ligands metals Protein-protein interface(s) links
Hydrolase PDB id
3tg0
Jmol
Contents
Protein chains
440 a.a.
Ligands
PO4 ×4
Metals
_ZN ×8
_MG ×4
Waters ×1878
PDB id:
3tg0
Name: Hydrolase
Title: E. Coli alkaline phosphatase with bound inorganic phosphate
Structure: Alkaline phosphatase. Chain: a, b, c, d. Fragment: unp residues 23-471. Synonym: apase. Engineered: yes
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: phoa, b0383, jw0374. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
1.20Å     R-factor:   0.149     R-free:   0.169
Authors: E.Bobyr,J.K.Lassila,H.I.Wiersma-Koch,T.D.Fenn,J.J.Lee,I.Niko Hughes,K.O.Hodgson,D.C.Rees,B.Hedman,D.Herschlag
Key ref: E.Bobyr et al. (2012). High-resolution analysis of Zn(2+) coordination in the alkaline phosphatase superfamily by EXAFS and x-ray crystallography. J Mol Biol, 415, 102-117. PubMed id: 22056344 DOI: 10.1016/j.jmb.2011.10.040
Date:
16-Aug-11     Release date:   09-Nov-11    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P00634  (PPB_ECOLI) -  Alkaline phosphatase
Seq:
Struc:
471 a.a.
440 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.3.1.3.1  - Alkaline phosphatase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: A phosphate monoester + H2O = an alcohol + phosphate
phosphate monoester
+ H(2)O
= alcohol
+
phosphate
Bound ligand (Het Group name = PO4)
corresponds exactly
      Cofactor: Mg(2+); Zn(2+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     periplasmic space   2 terms 
  Biological process     metabolic process   3 terms 
  Biochemical function     catalytic activity     10 terms  

 

 
    reference    
 
 
DOI no: 10.1016/j.jmb.2011.10.040 J Mol Biol 415:102-117 (2012)
PubMed id: 22056344  
 
 
High-resolution analysis of Zn(2+) coordination in the alkaline phosphatase superfamily by EXAFS and x-ray crystallography.
E.Bobyr, J.K.Lassila, H.I.Wiersma-Koch, T.D.Fenn, J.J.Lee, I.Nikolic-Hughes, K.O.Hodgson, D.C.Rees, B.Hedman, D.Herschlag.
 
  ABSTRACT  
 
No abstract given.