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PDBsum entry 3t2j

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protein ligands metals links
Hydrolase PDB id
3t2j
Jmol
Contents
Protein chain
316 a.a.
Ligands
BET ×6
DMS
Metals
_CA ×4
_ZN ×3
_CL ×2
_NA
Waters ×350
PDB id:
3t2j
Name: Hydrolase
Title: Tetragonal thermolysin in the presence of betaine
Structure: Thermolysin. Chain: e. Synonym: thermostable neutral proteinase. Ec: 3.4.24.27
Source: Bacillus thermoproteolyticus. Organism_taxid: 1427
Resolution:
2.00Å     R-factor:   0.162     R-free:   0.188
Authors: J.Cahn,N.S.Hti Lar Seng,D.Juers
Key ref: H.Marshall et al. (2012). The use of trimethylamine N-oxide as a primary precipitating agent and related methylamine osmolytes as cryoprotective agents for macromolecular crystallography. Acta Crystallogr D Biol Crystallogr, 68, 69-81. PubMed id: 22194335 DOI: 10.1107/S0907444911050360
Date:
22-Jul-11     Release date:   28-Dec-11    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P00800  (THER_BACTH) -  Thermolysin
Seq:
Struc:
 
Seq:
Struc:
548 a.a.
316 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 2 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.3.4.24.27  - Thermolysin.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Preferential cleavage: Xaa-|-Leu > Xaa-|-Phe.
      Cofactor: Ca(2+); Zn(2+)
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     proteolysis   1 term 
  Biochemical function     metalloendopeptidase activity     1 term  

 

 
DOI no: 10.1107/S0907444911050360 Acta Crystallogr D Biol Crystallogr 68:69-81 (2012)
PubMed id: 22194335  
 
 
The use of trimethylamine N-oxide as a primary precipitating agent and related methylamine osmolytes as cryoprotective agents for macromolecular crystallography.
H.Marshall, M.Venkat, N.S.Seng, J.Cahn, D.H.Juers.
 
  ABSTRACT  
 
No abstract given.