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PDBsum entry 3sat

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protein dna_rna ligands metals links
Hydrolase/DNA PDB id
3sat
Jmol
Contents
Protein chain
251 a.a.
DNA/RNA
Ligands
GOL
Metals
_ZN
Waters ×155
PDB id:
3sat
Name: Hydrolase/DNA
Title: Mutm slanted complex 6 with r112a mutation
Structure: DNA glycosylase. Chain: a. Engineered: yes. Mutation: yes. 5'-d( Ap Gp G Tp Ap Gp Ap Cp Cp Ap Gp Gp Ap Cp Gp chain: b. Engineered: yes. 5'-d( Tp Gp Cp Gp T Cp Cp Tp Gp Gp (Tx2) p Cp Tp 3'.
Source: Geobacillus stearothermophilus. Bacillus thermoliquefaciens. Organism_taxid: 1422. Gene: mutm. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Synthetic: yes
Resolution:
2.15Å     R-factor:   0.189     R-free:   0.225
Authors: Y.Qi,G.L.Verdine
Key ref: Y.Qi et al. (2012). Strandwise translocation of a DNA glycosylase on undamaged DNA. Proc Natl Acad Sci U S A, 109, 1086-1091. PubMed id: 22219368 DOI: 10.1073/pnas.1111237108
Date:
03-Jun-11     Release date:   11-Jan-12    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P84131  (P84131_GEOSE) -  Formamidopyrimidine-DNA glycosylase
Seq:
Struc:
274 a.a.
251 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 3 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.3.2.2.23  - DNA-formamidopyrimidine glycosylase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Hydrolysis of DNA containing ring-opened N(7)-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimide.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     metabolic process   6 terms 
  Biochemical function     catalytic activity     12 terms  

 

 
DOI no: 10.1073/pnas.1111237108 Proc Natl Acad Sci U S A 109:1086-1091 (2012)
PubMed id: 22219368  
 
 
Strandwise translocation of a DNA glycosylase on undamaged DNA.
Y.Qi, K.Nam, M.C.Spong, A.Banerjee, R.J.Sung, M.Zhang, M.Karplus, G.L.Verdine.
 
  ABSTRACT  
 
No abstract given.