PDBsum entry 3rvb

Hydrolase/hydrolase inhibitor

PDB id: 3rvb

Contents

Protein chain

443 a.a.

Ligands

I79

Metals

_CL

_MG ×2

Waters ×131

PDB id:

3rvb

Name:

Hydrolase/hydrolase inhibitor

Title:

The structure of hcv ns3 helicase (heli-80) bound with inhibitor itmn- 3479

Structure:

RNA helicase. Chain: a. Fragment: unp residues 1186-1658. Engineered: yes

Source:

Hepatitis c virus. Hcv. Organism_taxid: 11103. Gene: ns3. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.20Å R-factor: 0.227 R-free: 0.287

Authors:

O.Mather,R.Cheng,D.Schonfield,J.Barker

Key ref:

O.Mather et al. The structure of hcv ns3 helicase with inhibitor itmn. To be published, .

Date:

06-May-11 Release date: 24-Sep-14

Protein chain

P26663  (POLG_HCVBK) -  Genome polyprotein from Hepatitis C virus genotype 1b (isolate BK)

Seq: 3010 a.a.

Struc: 443 a.a.*

* PDB and UniProt seqs differ at 16 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: E.C.2.7.7.48 - RNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
• Enzyme class 2: E.C.3.4.21.98 - hepacivirin.
• Reaction: Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
• Enzyme class 3: E.C.3.4.22.- - ?????
• Enzyme class 4: E.C.3.6.1.15 - nucleoside-triphosphate phosphatase.
• Reaction: a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H⁺
• Enzyme class 5: E.C.3.6.4.13 - Rna helicase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site