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PDBsum entry 3ru4

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protein ligands metals Protein-protein interface(s) links
Hydrolase/hydrolase inhibitor PDB id
3ru4
Jmol
Contents
Protein chains
223 a.a.
61 a.a.
11 a.a.
131 a.a.
96 a.a.
Ligands
GOL ×6
SO4 ×9
EDO ×6
MRD ×2
Metals
_CA
Waters ×694
PDB id:
3ru4
Name: Hydrolase/hydrolase inhibitor
Title: Crystal structure of the bowman-birk serine protease inhibit complex with trypsin and chymotrypsin
Structure: Cationic trypsin. Chain: t. Synonym: beta-trypsin, alpha-trypsin chain 1, alpha-trypsin bowman-birk type seed trypsin and chymotrypsin in chain: b. Synonym: btci. Chymotrypsinogen a. Chain: c. Synonym: chymotrypsin a chain a.
Source: Bos taurus. Bovine,cow,domestic cattle,domestic cow. Organism_taxid: 9913. Vigna unguiculata. Cowpea. Organism_taxid: 3917. Organism_taxid: 9913
Resolution:
1.68Å     R-factor:   0.159     R-free:   0.192
Authors: G.F.Esteves,C.R.Santos,M.M.Ventura,J.A.R.G.Barbosa,S.M.Freit
Key ref: G.F.Esteves et al. Crystal structure of the bowman-Birk serine protease inhibitor btci in complex with trypsin and chymotryps. To be published, .
Date:
04-May-11     Release date:   29-Aug-12    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P00760  (TRY1_BOVIN) -  Cationic trypsin
Seq:
Struc:
246 a.a.
223 a.a.
Protein chain
Pfam   ArchSchema ?
P17734  (IBB_VIGUN) -  Bowman-Birk type seed trypsin and chymotrypsin inhibitor
Seq:
Struc:
83 a.a.
61 a.a.*
Protein chain
Pfam   ArchSchema ?
P00766  (CTRA_BOVIN) -  Chymotrypsinogen A
Seq:
Struc:
245 a.a.
11 a.a.
Protein chain
Pfam   ArchSchema ?
P00766  (CTRA_BOVIN) -  Chymotrypsinogen A
Seq:
Struc:
245 a.a.
131 a.a.
Protein chain
Pfam   ArchSchema ?
P00766  (CTRA_BOVIN) -  Chymotrypsinogen A
Seq:
Struc:
245 a.a.
96 a.a.
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 9 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class 2: Chains C, D, E: E.C.3.4.21.1  - Chymotrypsin.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa.
   Enzyme class 3: Chain T: E.C.3.4.21.4  - Trypsin.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   2 terms 
  Biological process     digestion   2 terms 
  Biochemical function     catalytic activity     8 terms