spacer
spacer

PDBsum entry 3rt1

Go to PDB code: 
protein ligands Protein-protein interface(s) links
Transferase PDB id
3rt1
Jmol
Contents
Protein chains
638 a.a.
Ligands
G6P ×5
GLC-GLC-GLC-GLC-
GLC-GLC-GLC-GLC
GLC-GLC-GLC
PEG ×4
GLC-GLC-GLC-GLC
GLC-GLC-GLC-GLC-
GLC
×2
PDB id:
3rt1
Name: Transferase
Title: Maltodextarn bound activated state form of yeast glycogen sy isoform 2
Structure: Protein (glycogen [starch] synthase isoform 2). Chain: a, b, c, d. Engineered: yes. Mutation: yes
Source: Saccharomyces cerevisiae. Brewer's yeast,lager beer yeast,yeast. Organism_taxid: 4932. Gene: gsy2, l8479.8, ylr258w. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Resolution:
2.80Å     R-factor:   0.213     R-free:   0.261
Authors: S.Baskaran,T.D.Hurley
Key ref: S.Baskaran et al. (2011). Multiple glycogen-binding sites in eukaryotic glycogen synthase are required for high catalytic efficiency toward glycogen. J Biol Chem, 286, 33999-34006. PubMed id: 21835915 DOI: 10.1074/jbc.M111.264531
Date:
02-May-11     Release date:   10-Aug-11    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P27472  (GYS2_YEAST) -  Glycogen [starch] synthase isoform 2
Seq:
Struc:
 
Seq:
Struc:
705 a.a.
638 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 3 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.2.4.1.11  - Glycogen(starch) synthase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
Glycogen
      Reaction: UDP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha- D-glucosyl)(n+1)
UDP-alpha-D-glucose
+ ((1->4)-alpha-D-glucosyl)(n)
Bound ligand (Het Group name = G6P)
matches with 46.43% similarity
= UDP
+ ((1->4)-alpha- D-glucosyl)(n+1)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cytoplasm   1 term 
  Biological process     metabolic process   2 terms 
  Biochemical function     catalytic activity     4 terms  

 

 
    Added reference    
 
 
DOI no: 10.1074/jbc.M111.264531 J Biol Chem 286:33999-34006 (2011)
PubMed id: 21835915  
 
 
Multiple glycogen-binding sites in eukaryotic glycogen synthase are required for high catalytic efficiency toward glycogen.
S.Baskaran, V.M.Chikwana, C.J.Contreras, K.D.Davis, W.A.Wilson, A.A.DePaoli-Roach, P.J.Roach, T.D.Hurley.
 
  ABSTRACT  
 
No abstract given.

 

 

spacer

spacer