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PDBsum entry 3rpt

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protein ligands Protein-protein interface(s) links
Hydrolase PDB id
3rpt
Jmol
Contents
Protein chains
252 a.a.
Ligands
SO4
Waters ×357
PDB id:
3rpt
Name: Hydrolase
Title: Crystal structure of the anti-HIV b12 scaffold protein
Structure: Endoglucanase e-2. Chain: x, a. Synonym: cellulase e-2, cellulase e2, endo-1,4-beta-glucana engineered: yes
Source: Thermobifida fusca. Organism_taxid: 2021. Gene: celb. Expressed in: escherichia coli. Expression_system_taxid: 562
Resolution:
1.30Å     R-factor:   0.175     R-free:   0.202
Authors: L.Chen,P.D.Kwong
Key ref: M.L.Azoitei et al. (2011). Computation-guided backbone grafting of a discontinuous motif onto a protein scaffold. Science, 334, 373-376. PubMed id: 22021856 DOI: 10.1126/science.1209368
Date:
27-Apr-11     Release date:   21-Dec-11    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P26222  (GUN2_THEFU) -  Endoglucanase E-2
Seq:
Struc:
441 a.a.
252 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 21 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.3.2.1.4  - Cellulase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endohydrolysis of 1,4-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     cellulose catabolic process   1 term 
  Biochemical function     hydrolase activity, hydrolyzing O-glycosyl compounds     1 term  

 

 
DOI no: 10.1126/science.1209368 Science 334:373-376 (2011)
PubMed id: 22021856  
 
 
Computation-guided backbone grafting of a discontinuous motif onto a protein scaffold.
M.L.Azoitei, B.E.Correia, Y.E.Ban, C.Carrico, O.Kalyuzhniy, L.Chen, A.Schroeter, P.S.Huang, J.S.McLellan, P.D.Kwong, D.Baker, R.K.Strong, W.R.Schief.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
23135467 N.Koga, R.Tatsumi-Koga, G.Liu, R.Xiao, T.B.Acton, G.T.Montelione, and D.Baker (2012).
Principles for designing ideal protein structures.
  Nature, 491, 222-227.
PDB codes: 2kl8 2ln3 2lta 2lv8 2lvb
22113616 J.S.McLellan, M.Pancera, C.Carrico, J.Gorman, J.P.Julien, R.Khayat, R.Louder, R.Pejchal, M.Sastry, K.Dai, S.O'Dell, N.Patel, S.Shahzad-ul-Hussan, Y.Yang, B.Zhang, T.Zhou, J.Zhu, J.C.Boyington, G.Y.Chuang, D.Diwanji, I.Georgiev, Y.D.Kwon, D.Lee, M.K.Louder, S.Moquin, S.D.Schmidt, Z.Y.Yang, M.Bonsignori, J.A.Crump, S.H.Kapiga, N.E.Sam, B.F.Haynes, D.R.Burton, W.C.Koff, L.M.Walker, S.Phogat, R.Wyatt, J.Orwenyo, L.X.Wang, J.Arthos, C.A.Bewley, J.R.Mascola, G.J.Nabel, W.R.Schief, A.B.Ward, I.A.Wilson, and P.D.Kwong (2011).
Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9.
  Nature, 480, 336-343.
PDB codes: 3tcl 3u1s 3u2s 3u36 3u46 3u4b 3u4e
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